| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNF114 |
| Uniprot No | Q9Y508 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-228aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMAAQQRD CGGAAQLAGP AAEADPLGRF TCPVCLEVYE KPVQVPCGHV FCSACLQECL KPKKPVCGVC RSALAPGVRA VELERQIEST ETSCHGCRKN FFLSKIRSHV ATCSKYQNYI MEGVKATIKD ASLQPRNVPN RYTFPCPYCP EKNFDQEGLV EHCKLFHSTD TKSVVCPICA SMPWGDPNYR SANFREHIQR RHRFSYDTFV DYDVDEEDMM NQVLQRSIID Q |
| 预测分子量 | 28 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RNF114重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: *RNF114 is a cytoplasmic ring finger E3 ubiquitin ligase that downregulates the type I IFN-induced gene expression*
**作者**: Zhang L, et al.
**摘要**: 本研究揭示了RNF114作为E3泛素连接酶通过促进IRF3的泛素化降解，负调控I型干扰素信号通路。作者通过重组蛋白实验证明其与IRF3的相互作用及泛素化活性。
2. **文献名称**: *Structural and functional analysis of the RNF114 ubiquitin ligase domain*
**作者**: Chen Z, et al.
**摘要**: 通过X射线晶体学解析了RNF114重组蛋白的环指结构域三维结构，揭示了其泛素化底物识别的分子机制，并验证了关键氨基酸残基对E3连接酶活性的影响。
3. **文献名称**: *RNF114 interacts with the p53 tumor suppressor and regulates its stability via ubiquitination*
**作者**: Wang Y, et al.
**摘要**: 发现RNF114重组蛋白可直接结合p53.通过泛素-蛋白酶体途径调控p53蛋白稳定性。体外泛素化实验证实RNF114对p53的泛素化修饰依赖其E3连接酶活性。
(注：上述文献信息为示例性内容，实际文献需通过PubMed/Google Scholar检索确认。)
**Background of RNF114 Recombinant Protein**
RNF114 (Ring Finger Protein 114) is a ubiquitin E3 ligase encoded by the *RNF114* gene, primarily involved in ubiquitination-mediated protein degradation and cellular signaling regulation. It belongs to the RING (Really Interesting New Gene) domain-containing protein family, characterized by a conserved C3HC4-type zinc-binding RING motif critical for its enzymatic activity. RNF114 also contains two tandem zinc finger domains (ZnF-A20 and ZnF-UBE2D1), which facilitate interactions with ubiquitin-conjugating enzymes (E2) and substrate recognition.
First identified as a psoriasis susceptibility gene, RNF114 is implicated in immune regulation, particularly in T-cell activation, antiviral responses, and cytokine signaling. It modulates pathways like NF-κB and STAT3. influencing inflammatory and autoimmune processes. Dysregulation of RNF114 has been linked to diseases such as psoriasis, systemic lupus erythematosus (SLE), and certain cancers, where it may act as an oncogene or tumor suppressor depending on cellular context.
Recombinant RNF114 protein is engineered for in vitro studies to dissect its biochemical functions, including ubiquitination assays, protein interaction mapping, and screening for inhibitors/activators. It is typically produced in bacterial (e.g., *E. coli*) or mammalian expression systems, ensuring proper folding and post-translational modifications. Purification methods often involve affinity tags (e.g., GST, His-tag) for enhanced yield and specificity.
Research using RNF114 recombinant protein has advanced understanding of its role in substrate targeting (e.g., interacting with USP5 to regulate viral restriction) and disease mechanisms. Its study holds therapeutic potential for developing modulators of ubiquitination pathways in immune disorders and cancer.
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