| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RND3 |
| Uniprot No | P61587 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-241aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMKERRASQKLSSKSIMDPNQNVKCKIVVVG DSQCGKTALLHVFAKDCFPENYVPTVFENYTASFEIDTQRIELSLWDTSG SPYYDNVRPLSYPDSDAVLICFDISRPETLDSVLKKWKGEIQEFCPNTKM LLVGCKSDLRTDVSTLVELSNHRQTPVSYDQGANMAKQIGAATYIECSAL QSENSVRDIFHVATLACVNKTNKNVKRNKSQRATKRISHMPSRPELSAVA TDLRKDKAKSC |
| 预测分子量 | 29 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RND3重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"RND3 regulates smooth muscle cell migration via nuclear translocation and actin reorganization"*
**作者**: Li et al. (2017)
**摘要内容**: 该研究探讨了RND3重组蛋白在平滑肌细胞迁移中的作用。通过体外重组表达RND3.发现其通过促进核转位和调节肌动蛋白细胞骨架重组，抑制细胞迁移，提示RND3在血管疾病中的潜在治疗价值。
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2. **文献名称**: *"Recombinant RND3/RhoE induces apoptosis by modulating the p53 pathway in cancer cells"*
**作者**: Zhang et al. (2015)
**摘要内容**: 研究利用大肠杆菌系统表达纯化RND3重组蛋白，验证其通过激活p53依赖的凋亡通路抑制肿瘤细胞增殖，为靶向RND3的癌症治疗策略提供了实验依据。
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3. **文献名称**: *"Structural and functional characterization of the RND3 GTPase domain"*
**作者**: Wang et al. (2020)
**摘要内容**: 该文献通过X射线晶体学解析了RND3重组蛋白的GTP酶结构域三维结构，揭示了其非典型GTP结合特性及与下游效应蛋白的相互作用机制，为理解RND3在细胞信号转导中的功能提供了结构基础。
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**备注**：以上文献信息为示例，实际文献需通过PubMed或Google Scholar检索确认。建议使用关键词“RND3 recombinant protein”、“RhoE expression”或结合具体研究领域(如癌症、细胞迁移)进行精确查找。
**Background of RND3 Recombinant Protein**
RND3 (Rho Family GTPase 3), also known as RhoE, is a member of the Rho GTPase family, which regulates cytoskeletal dynamics, cell motility, and signaling pathways. Unlike classical Rho GTPases (e.g., RhoA, Rac1), RND3 lacks intrinsic GTPase activity, functioning as a constitutively active GTP-bound protein. It acts as a key modulator of actin cytoskeleton organization, antagonizing RhoA/ROCK-mediated stress fiber formation and promoting cell rounding or migration. RND3 also interacts with critical signaling pathways, including PI3K/AKT and MAPK, influencing cell proliferation, differentiation, and apoptosis.
Recombinant RND3 protein is engineered in vitro using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional RND3 for research. This protein typically retains post-translational modifications when expressed in mammalian systems, enhancing its biological relevance. Researchers utilize RND3 recombinant protein to study its role in cellular processes, such as cancer metastasis, neuronal development, and vascular remodeling. For instance, RND3 is implicated in tumor suppression by inhibiting invasion in certain cancers (e.g., glioblastoma) but may exhibit oncogenic properties in others, highlighting its context-dependent functionality.
In neurodegenerative diseases, RND3 regulates axonal guidance and synaptic plasticity, while in cardiovascular systems, it influences endothelial barrier integrity. The recombinant form enables mechanistic studies, protein interaction assays, and therapeutic screening. Its applications extend to understanding RND3’s interplay with other GTPases and developing targeted therapies for diseases linked to cytoskeletal dysregulation. Overall, RND3 recombinant protein serves as a vital tool for dissecting the complex roles of Rho GTPases in health and disease.
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