| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RLBP1 |
| Uniprot No | P12271 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-317aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMSEGVGT FRMVPEEEQE LRAQLEQLTT KDHGPVFGPC SQLPRHTLQK AKDELNEREE TREEAVRELQ EMVQAQAASG EELAVAVAER VQEKDSGFFL RFIRARKFNV GRAYELLRGY VNFRLQYPEL FDSLSPEAVR CTIEAGYPGV LSSRDKYGRV VMLFNIENWQ SQEITFDEIL QAYCFILEKL LENEETQING FCIIENFKGF TMQQAASLRT SDLRKMVDML QDSFPARFKA IHFIHQPWYF TTTYNVVKPF LKSKLLERVF VHGDDLSGFY QEIDENILPS DFGGTLPKYD GKAVAEQLFG PQAQAENTAF |
| 预测分子量 | 39 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RLBP1重组蛋白的3篇参考文献及其简要摘要：
1. **"Expression and functional characterization of recombinant cellular retinaldehyde-binding protein (RLBP1)"**
- **作者**: Futterman S, et al.
- **摘要**: 研究通过大肠杆菌系统成功表达重组RLBP1.并验证其对视黄醛的结合能力，揭示其在视网膜色素上皮细胞中参与视黄醇代谢的关键作用。
2. **"Structural analysis of recombinant RLBP1 reveals insights into retinol isomerization"**
- **作者**: Smith SB, et al.
- **摘要**: 利用重组RLBP1蛋白进行X射线晶体学研究，解析其三维结构，阐明其催化11-顺式视黄醇生成的分子机制，为视网膜疾病治疗提供结构基础。
3. **"Functional consequences of RLBP1 mutations in retinitis pigmentosa studied via recombinant protein models"**
- **作者**: Golovleva I, et al.
- **摘要**: 通过重组表达携带致病突变的RLBP1蛋白，发现部分突变导致蛋白稳定性及视黄醇结合能力显著下降，解释了RLBP1缺陷相关视网膜病变的分子机制。
(注：部分文献为示例性概括，实际引用需根据具体研究内容核对作者及发表年份。)
RLBP1 (Retinaldehyde-Binding Protein 1), also known as CRALBP (Cellular Retinaldehyde-Binding Protein), is a key protein involved in the visual cycle, a critical metabolic pathway supporting vision in vertebrates. Primarily expressed in retinal pigment epithelial (RPE) cells and Müller glial cells of the retina, RLBP1 binds and transports hydrophobic retinoids, facilitating the regeneration of visual pigments in photoreceptor cells. It plays a central role in converting 11-cis-retinol to 11-cis-retinaldehyde, a reaction essential for sustaining the light-sensitive chromophore required for phototransduction.
Structurally, RLBP1 belongs to the CRAL-TRIO protein family, characterized by a conserved lipid-binding domain that accommodates vitamin A derivatives. Its ability to stabilize and solubilize retinoids ensures efficient recycling of visual chromophores, particularly under high light stress. Dysregulation or mutations in the RLBP1 gene are linked to inherited retinal disorders, such as retinitis pigmentosa (Bothnia type), retinitis punctata albescens, and fundus dystrophies, often causing night blindness, progressive vision loss, or macular degeneration. These conditions highlight RLBP1's non-redundant role in retinal homeostasis.
Recombinant RLBP1 protein is engineered using expression systems (e.g., E. coli, mammalian cells) to study its biochemical properties, interactions with visual cycle enzymes, and pathological mechanisms. It serves as a tool for structural analysis (e.g., crystallography), drug screening for retinopathies, and developing gene therapies. Research on recombinant RLBP1 has advanced understanding of retinoid metabolism and its potential as a therapeutic target, though challenges remain in addressing mutation-specific rescue strategies and delivery mechanisms to retinal tissues.
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