| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RhoG |
| Uniprot No | P84095 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-188aa |
| 氨基酸序列 | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMQS IKCVVVGDGA VGKTCLLICY TTNAFPKEYI PTVFDNYSAQ SAVDGRTVNL NLWDTAGQEE YDRLRTLSYP QTNVFVICFS IASPPSYENV RHKWHPEVCH HCPDVPILLV GTKKDLRAQP DTLRRLKEQG QAPITPQQGQ ALAKQIHAVR YLECSALQQD GVKEVFAEAV RAVLNPTPIK RGRSC |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RhoG重组蛋白的3篇代表性文献的简要信息(注：文献为模拟示例，具体内容需根据实际文献调整)：
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1. **文献名称**: *"RhoG activates Rac1 and regulates phagocytosis through the ELMO/DOCK180 complex"*
**作者**: Katoh H. et al.
**摘要**: 研究揭示了RhoG重组蛋白在吞噬作用中的关键作用，通过与ELMO/DOCK180复合物结合激活Rac1信号通路，促进细胞骨架重组和病原体清除。
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2. **文献名称**: *"Recombinant RhoG regulates epithelial cell migration via integrin signaling"*
**作者**: García-Mata R. et al.
**摘要**: 利用重组RhoG蛋白证明其通过整合素依赖性途径调控上皮细胞迁移，并发现RhoG与β1整合素的互作对肿瘤细胞侵袭能力的影响。
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3. **文献名称**: *"Expression and purification of active RhoG GTPase in E. coli for structural studies"*
**作者**: Smith J.L. & Zheng Y.
**摘要**: 报道了一种在大肠杆菌中高效表达并纯化功能性RhoG重组蛋白的方法，通过体外GTPase活性实验验证其构象变化，为结构生物学研究提供基础。
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(提示：实际文献可通过PubMed或Google Scholar以关键词“RhoG recombinant protein”或“RhoG GTPase function”检索获取。)
RhoG (Ras homolog family member G) is a small GTP-binding protein belonging to the Rho GTPase family, which plays a central role in regulating cytoskeletal dynamics, cell migration, and membrane trafficking. Discovered in the 1990s, RhoG shares structural and functional similarities with other Rho family members like Rac1 and Cdc42. operating as a molecular switch by cycling between active GTP-bound and inactive GDP-bound states. Its activity is tightly regulated by guanine nucleotide exchange factors (GEFs), GTPase-activating proteins (GAPs), and guanine nucleotide dissociation inhibitors (GDIs). RhoG is notably involved in signaling pathways that control cell adhesion, neurite outgrowth, and phagocytosis, often interacting with downstream effectors such as ELMO and Dock180 to activate Rac1. thereby linking RhoG to broader cellular processes.
Recombinant RhoG proteins are engineered using heterologous expression systems (e.g., E. coli or mammalian cells) to produce purified, functional proteins for research. These tools enable studies on RhoG’s structure-function relationships, activation mechanisms, and interactions with regulatory molecules. Recombinant RhoG is particularly valuable in dissecting pathways implicated in cancer metastasis, immune cell migration, and neurological disorders, where dysregulated Rho GTPase signaling is frequently observed. For example, aberrant RhoG expression has been linked to invasive tumor behavior and impaired neuronal development. Additionally, recombinant RhoG serves as a critical reagent in drug discovery, aiding the identification of inhibitors or activators targeting Rho GTPase-related pathologies. Its applications extend to in vitro assays, structural studies (e.g., crystallography), and cellular models to explore RhoG’s role in health and disease.
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