| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RhoB |
| Uniprot No | P62745 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-193aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMAAIRKKLVVVGDGACGKTCLLIVFSKDEF PEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVI LMCFSVDSPDSLENIPEKWVPEVKHFCPNVPIILVANKKDLRSDEHVRTE LARMKQEPVRTDDGRAMAVRIQAYDYLECSAKTKEGVREVFETATRAALQ KRYGSQNGCINCC |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RhoB重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*"RhoB regulates cancer cell apoptosis through interaction with Bcl-2 family proteins"*
**作者**：Peng, X. et al. (2015)
**摘要**：该研究通过重组RhoB蛋白实验，发现其与Bcl-2家族蛋白的相互作用可调控癌细胞凋亡，尤其在卵巢癌中，RhoB过表达能增强化疗药物的敏感性。
2. **文献名称**：*"Recombinant RhoB expression in E. coli: Purification and functional characterization in cytoskeleton remodeling"*
**作者**：Smith, J.R. et al. (2012)
**摘要**：作者利用大肠杆菌系统高效表达并纯化重组RhoB蛋白，证实其在体外能够激活下游效应分子(如ROCK)，并参与细胞骨架重排的调控机制。
3. **文献名称**：*"RhoB mediates DNA damage response by modulating p53 activity in recombinant protein-based assays"*
**作者**：Fujii, K. et al. (2013)
**摘要**：通过重组RhoB蛋白与p53的共表达实验，研究发现RhoB通过稳定p53蛋白增强DNA损伤后的细胞周期阻滞和凋亡，揭示了其在肿瘤抑制中的新机制。
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**备注**：以上文献为示例性内容，实际引用时需核实具体来源及期刊信息。如需真实文献，建议通过PubMed或Web of Science以关键词“RhoB recombinant protein”检索近期研究。
RhoB, a member of the Rho GTPase family, regulates diverse cellular processes, including cytoskeleton dynamics, vesicular trafficking, and stress responses. Unlike other Rho proteins (e.g., RhoA, Rac1), RhoB exhibits unique characteristics: it localizes to endosomes, nuclear membranes, and plasma membranes, and shows rapid turnover due to its short half-life. Its expression is tightly controlled by cellular stress signals, such as DNA damage, hypoxia, or growth factor stimulation, linking it to apoptosis, autophagy, and cancer progression.
Recombinant RhoB protein is produced via heterologous expression systems (e.g., E. coli, mammalian cells) to study its biochemical properties and signaling mechanisms. Purification often involves affinity tags (e.g., GST, His-tag) for enhanced yield and specificity. While bacterial systems offer cost-effective production, they lack post-translational modifications (e.g., prenylation) critical for RhoB membrane association. Eukaryotic systems (e.g., insect or mammalian cells) better mimic native lipid modifications, preserving functional activity.
Research applications include investigating RhoB’s role in cancer, where it acts as a tumor suppressor by opposing oncogenic RhoA/C signals. It regulates metastasis, angiogenesis, and chemoresistance, making it a potential therapeutic target or biomarker. Recombinant RhoB facilitates in vitro studies, such as GTPase activity assays, effector protein binding (e.g., ROCK, PI3K), and drug screening for Rho pathway modulators. Structural studies using recombinant protein also aid in understanding its unique regulatory domains and interaction interfaces. Despite its functional complexity, recombinant RhoB remains a vital tool for unraveling its context-dependent roles in cellular homeostasis and disease.
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