| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RGS2 |
| Uniprot No | P41220 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-211aa |
| 氨基酸序列 | MQSAMFLAVQHDCRPMDKSAGSGHKSEEKREKMKRTLLKDWKTRLSYFLQNSSTPGKPKTGKKSKQQAFIKPSPEEAQLWSEAFDELLASKYGLAAFRAFLKSEFCEENIEFWLACEDFKKTKSPQKLSSKARKIYTDFIEKEAPKEINIDFQTKTLIAQNIQEATSGCFTTAQKRVYSLMENNSYPRFLESEFYQDLCKKPQITTEPHAT |
| 预测分子量 | 40.4kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Regulation of G-Protein Signaling by RGS2: Biochemical and Structural Analysis"**
*作者：Berman, D. M., et al.*
摘要：该研究通过重组表达纯化RGS2蛋白，分析其与Gα亚基的相互作用机制，揭示其加速GTP水解的分子基础，证实RGS2作为GAP(GTP酶激活蛋白)的功能。
2. **"RGS2 Selectively Regulates Gαq Signaling in Vitro and in Vivo"**
*作者：Heximer, S. P., et al.*
摘要：利用重组RGS2蛋白进行体外结合实验，证明其对Gαq亚基的特异性抑制，并探讨其在心血管系统中调控血压的生理意义。
3. **"Crystal Structure of RGS2 in Complex with Gαi1"**
*作者：Zhong, H., & Neubig, R. R.*
摘要：通过X射线晶体学解析重组RGS2与Gαi1的复合物结构，阐明其结合界面及调控G蛋白信号传导的空间构象变化。
4. **"Phosphorylation-Dependent Regulation of RGS2 Activity"**
*作者：Salim, S., et al.*
摘要：研究重组RGS2蛋白的磷酸化修饰(如PKC介导的修饰)对其与Gαq结合能力的影响，揭示翻译后修饰在信号通路动态调控中的作用。
**Background of RGS2 Recombinant Protein**
Regulator of G-protein signaling 2 (RGS2) is a member of the RGS protein family, which plays a critical role in modulating G-protein-coupled receptor (GPCR) signaling pathways. RGS proteins act as GTPase-activating proteins (GAPs) for Gα subunits, accelerating the hydrolysis of GTP to GDP, thereby terminating GPCR-mediated signals. RGS2 specifically targets Gαq/11 subunits, making it a key regulator of pathways involving phospholipase C (PLC) activation, calcium mobilization, and downstream physiological responses.
RGS2 is ubiquitously expressed, with notable roles in cardiovascular, neurological, and immune systems. It has been implicated in blood pressure regulation, vascular remodeling, and anxiety-related behaviors. Dysregulation of RGS2 is associated with hypertension, heart failure, and neuropsychiatric disorders, highlighting its therapeutic potential.
Recombinant RGS2 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to ensure high purity and functionality. This engineered protein retains the conserved RGS domain required for GAP activity, enabling researchers to study its interactions with Gα subunits, screen for modulators, or explore its role in disease models. Applications include *in vitro* assays, structural studies, and drug discovery, particularly for GPCR-targeted therapies. Recent studies also investigate RGS2's non-canonical functions, such as scaffolding or transcription regulation, broadening its biomedical relevance.
Overall, RGS2 recombinant protein serves as a vital tool for dissecting GPCR signaling mechanisms and developing precision therapeutics.
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