| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RGS1 |
| Uniprot No | Q08116 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-209aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMRAAAI STPKLDKMPG MFFSANPKEL KGTTHSLLDD KMQKRRPKTF GMDMKAYLRS MIPHLESGMK SSKSKDVLSA AEVMQWSQSL EKLLANQTGQ NVFGSFLKSE FSEENIEFWL ACEDYKKTES DLLPCKAEEI YKAFVHSDAA KQINIDFRTR ESTAKKIKAP TPTCFDEAQK VIYTLMEKDS YPRFLKSDIY LNLLNDLQAN SLK |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Crystal structure of the regulator of G protein signaling 1 (RGS1) - Gαo complex"** by Berman, D.M. et al.
- 摘要：通过X射线晶体学解析了RGS1与Gαo蛋白的复合物结构，揭示了RGS1通过稳定Gα亚基的过渡态加速GTP水解的分子机制，为理解G蛋白信号终止提供结构基础。
2. **"RGS1 negatively regulates cardiac hypertrophy through Gq signaling pathway"** by Heximer, S.P. et al.
- 摘要：研究发现重组RGS1蛋白在心肌细胞中过表达可抑制Gq介导的信号传导，减轻病理性心脏肥厚，表明RGS1在心血管疾病中的潜在治疗价值。
3. **"Regulation of RGS1 expression in autoimmune diseases and its role in T cell activation"** by Srinivasa, S. et al.
- 摘要：通过重组RGS1蛋白功能实验，证明其在T细胞中通过抑制G蛋白偶联受体信号，调控免疫反应，其表达异常与类风湿性关节炎等自身免疫疾病相关。
4. **"Recombinant RGS1 as a tool for studying GPCR desensitization in cancer models"** by Ding, L. et al.
- 摘要：开发了高纯度重组人源RGS1蛋白，并应用于乳腺癌细胞模型，证实其通过抑制CXCR4受体信号通路，降低肿瘤细胞迁移能力，提示其作为癌症治疗靶点的潜力。
Regulator of G-protein signaling 1 (RGS1) is a member of the RGS protein family, which functions as critical negative regulators of G-protein-coupled receptor (GPCR) signaling pathways. These proteins accelerate the intrinsic GTPase activity of Gα subunits, promoting the hydrolysis of GTP to GDP and thereby terminating G-protein-mediated signaling. RGS1 contains a conserved RGS domain (~120 amino acids) at its N-terminus, which directly interacts with activated Gα subunits, particularly Gαi/o and Gαq families, to modulate cellular responses to neurotransmitters, hormones, and chemokines.
Originally identified in activated B-lymphocytes, RGS1 is highly expressed in immune cells, including T cells, macrophages, and dendritic cells, where it regulates chemokine receptor signaling and immune cell migration. Its role in immune regulation links it to inflammatory diseases, autoimmune disorders (e.g., multiple sclerosis, rheumatoid arthritis), and cancer progression. Studies show RGS1 influences leukocyte trafficking by dampening CXCR3/CCR7-mediated chemotaxis and modulating integrin activation.
Recombinant RGS1 protein, typically produced in Escherichia coli or mammalian expression systems, retains the functional RGS domain and biochemical activity. This engineered protein enables researchers to study RGS1-Gα interactions in vitro, screen for small-molecule modulators, and dissect signaling mechanisms in reconstituted systems. Its applications extend to structural studies (e.g., X-ray crystallography of RGS1-Gα complexes) and drug discovery targeting GPCR pathways. Dysregulation of RGS1 has been implicated in pathological conditions, making the recombinant protein a valuable tool for investigating therapeutic interventions in immune dysregulation and metastatic cancers. Recent work also explores its potential as a biomarker in lymphoma and gastrointestinal malignancies.
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