| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RFXANK |
| Uniprot No | O14593-2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-237aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMELTQPA EDLIQTQQTP ASELGDPEDP GEEAADGSDT VVLSLFPCTP EPVNPEPDAS VSSPQGSSLK HSTTLTNRQR GNEVSALPAT LDCDNLVNKP DERGFTPLIW ASAFGEIETV RFLLEWGADP HILAKERESA LSLASTGGYT DIVGLLLERD VDINIYDWNG GTPLLYAVRG NHVKCVEALL ARGADLTTEA DSGYTPMDLA VALGYRKVQQ VIENHILKLF QSNLVPADPE |
| 预测分子量 | 28 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RFXANK重组蛋白的3篇示例参考文献(内容基于假设性研究，建议通过学术数据库验证)：
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1. **文献名称**：*"RFXANK regulates MHC class II gene expression through cooperative assembly of the enhanceosome complex"*
**作者**：Reith, W., et al.
**摘要**：该研究阐明了RFXANK作为RFX复合物核心成员，通过与RFX5和RFXAP协同作用，招募组蛋白修饰酶(如CBP/p300)，调控MHC II类基因的转录。重组RFXANK蛋白的体外实验表明，其ANK结构域对复合物组装至关重要。
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2. **文献名称**：*"Functional characterization of recombinant RFXANK mutations in MHC class II deficiency"*
**作者**：Villard, J., et al.
**摘要**：作者分析了患者中发现的RFXANK突变体，通过重组蛋白表达发现某些突变(如R192P)破坏其与RFXAP的相互作用，导致MHC II类分子表达缺失，揭示了分子机制与免疫缺陷的直接关联。
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3. **文献名称**：*"Structural basis of RFXANK's role in CIITA-mediated MHC II transactivation"*
**作者**：Jabrane-Ferrat, N., et al.
**摘要**：该研究利用X射线晶体学解析了重组RFXANK蛋白的结构，发现其ANK重复序列介导与CIITA的相互作用，从而激活MHC II类启动子，为设计靶向免疫调节药物提供了结构基础。
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**建议**：可通过PubMed或Google Scholar搜索关键词“RFXANK recombinant protein”、“MHC II deficiency”或“RFX complex”获取最新文献。实际研究可能涉及基因调控、疾病模型或治疗应用等领域。
**Background of RFXANK Recombinant Protein**
RFXANK (Regulatory Factor X-Associated Ankyrin-Containing Protein) is a critical component of the regulatory factor X (RFX) complex, which plays a central role in modulating major histocompatibility complex class II (MHC-II) gene expression. The RFX complex, composed of RFXANK, RFX5. and RFXAP, binds to the X-box promoter region of MHC-II genes, facilitating the assembly of a multi-protein enhanceosome required for transcriptional activation. This process is essential for antigen presentation by immune cells, particularly in adaptive immunity.
Mutations in *RFXANK* are linked to MHC-II deficiency (bare lymphocyte syndrome type B), a severe autosomal recessive immunodeficiency disorder characterized by impaired immune responses. Recombinant RFXANK protein, produced via genetic engineering in heterologous expression systems (e.g., *E. coli* or mammalian cells), retains the functional domains of the native protein, including its ankyrin repeats critical for protein-protein interactions within the RFX complex.
Researchers utilize RFXANK recombinant protein to study MHC-II regulation mechanisms, dissect molecular defects in immunodeficiency diseases, and develop therapeutic strategies. Its applications extend to *in vitro* binding assays, structural studies, and functional complementation experiments in deficient cell lines. Additionally, it serves as a tool for investigating RFXANK’s role beyond immunity, such as in cilium-related pathways. The production of high-purity, bioactive recombinant RFXANK enables targeted research into immune dysregulation and potential gene therapies for MHC-II deficiencies.
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