| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | REXO2 |
| Uniprot No | Q9Y3B8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 26-237aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSVREGGAAMAAGESMAQRMVWVDLEMTG LDIEKDQIIEMACLITDSDLNILAEGPNLIIKQPDELLDSMSDWCKEHHG KSGLTKAVKESTITLQQAEYEFLSFVRQQTPPGLCPLAGNSVHEDKKFLD KYMPQFMKHLHYRIIDVSTVKELCRRWYPEEYEFAPKKAASHRALDDISE SIKELQFYRNNIFKKKIDEKKRKIIENGENEKTVS |
| 预测分子量 | 27 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于REXO2重组蛋白的模拟参考文献示例(仅供参考，建议通过学术数据库获取真实文献)：
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1. **文献名称**: *Structural and Functional Characterization of Recombinant REXO2 Protein in RNA Processing*
**作者**: Müller, S. et al.
**摘要**: 本研究报道了人源REXO2重组蛋白的高效表达及纯化方法，并解析了其晶体结构。结果表明，REXO2通过外切酶活性参与线粒体RNA的3'末端加工，其重组蛋白在体外可有效降解单链RNA底物。
2. **文献名称**: *REXO2 Knockdown Alters Mitochondrial Gene Expression and Impairs Cell Viability*
**作者**: Tanaka, K. & Patel, R.
**摘要**: 通过构建REXO2重组蛋白突变体，研究证明其RNA外切酶活性对维持线粒体mRNA稳定性至关重要。敲低REXO2导致HeLa细胞能量代谢障碍，提示其在细胞呼吸链中的调控作用。
3. **文献名称**: *Optimization of Recombinant REXO2 Expression in E. coli for Enzymatic Assays*
**作者**: Zhang, Y. et al.
**摘要**: 本文优化了REXO2在大肠杆菌中的可溶性表达条件，通过亲和层析获得高纯度蛋白。酶动力学分析显示，重组REXO2对poly(A) RNA底物具有特异性水解活性，最适反应pH为8.0.
4. **文献名称**: *REXO2 Interaction with SUV3 Helicase in Mitochondrial RNA Surveillance*
**作者**: Garcia-Diaz, M. & Johansson, M.
**摘要**: 利用重组REXO2与SUV3蛋白的共表达实验，揭示了二者在线粒体RNA降解复合体中的协同作用。研究指出，REXO2的活性依赖SUV3的RNA解旋酶功能，共同维持RNA代谢稳态。
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**注意**：以上文献为模拟示例，实际研究请通过PubMed、Web of Science等平台检索。真实文献可能涉及更具体的机制或应用场景。
**Background of REXO2 Recombinant Protein**
REXO2 (RNA exonuclease 2) is a conserved eukaryotic enzyme belonging to the DEDDh family of 3’-5’ exonucleases. It plays a critical role in RNA metabolism, particularly in processing and degrading structured RNA substrates. REXO2 is homologous to the yeast protein Rex2p and is implicated in ribosomal RNA (rRNA) maturation, small non-coding RNA turnover, and mitochondrial RNA surveillance. Structurally, it contains a catalytic core with conserved acidic residues that coordinate metal ions (e.g., Mg²⁺ or Mn²⁺) essential for its exonuclease activity.
The recombinant REXO2 protein is engineered for in vitro studies to dissect its biochemical mechanisms. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells*), it is purified to homogeneity using affinity chromatography. Recombinant REXO2 enables researchers to analyze substrate specificity, cleavage kinetics, and interactions with cofactors like RNA helicases or polyadenylation complexes.
Biomedically, REXO2 dysfunction has been linked to mitochondrial disorders and cancer. Its role in maintaining RNA quality control makes it a potential therapeutic target. Recombinant REXO2 also serves as a tool for diagnostic assays, such as detecting RNA-processing defects. Recent studies highlight its involvement in antiviral defense by degrading viral RNA, expanding its relevance in immunology.
In summary, recombinant REXO2 provides a versatile platform for exploring RNA metabolism, disease mechanisms, and biotechnological applications.
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