| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RCVRN |
| Uniprot No | P35243 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-200aa |
| 氨基酸序列 | MGNSKSGALS KEILEELQLN TKFSEEELCS WYQSFLKDCP TGRITQQQFQ SIYAKFFPDT DPKAYAQHVF RSFDSNLDGT LDFKEYVIAL HMTTAGKTNQ KLEWAFSLYD VDGNGTISKN EVLEIVMAIF KMITPEDVKL LPDDENTPEK RAEKIWKYFG KNDDDKLTEK EFIEGTLANK EILRLIQFEP QKVKEKMKNA |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与RCVRN(Recoverin)重组蛋白相关的研究文献摘要示例：
1. **《Expression and purification of recombinant human recoverin in Escherichia coli》**
- 作者：Tao, X. et al.
- 摘要：研究报道了通过大肠杆菌表达系统高效表达人源重组Recoverin蛋白，优化纯化步骤获得高纯度蛋白，并通过圆二色谱验证其正确折叠和钙离子结合能力。
2. **《Structural insights into recoverin's calcium-myristoyl switch mechanism》**
- 作者：Flaherty, K.M. et al.
- 摘要：通过X射线晶体学解析重组Recoverin的钙离子依赖构象变化，揭示了其肉豆蔻酰基转移开关在视觉信号转导中的分子机制，解释了与视网膜光感受器功能的关系。
3. **《Autoantibody recognition of recombinant recoverin in cancer-associated retinopathy》**
- 作者：Maeda, T. et al.
- 摘要：利用重组Recoverin蛋白分析肿瘤相关性视网膜病变患者血清中的自身抗体反应，证实其作为疾病标志物的潜力，并探讨了Recoverin异常免疫反应与视网膜退化的关联。
注：以上文献为示例，实际引用时请核对真实文献来源及内容准确性。如需具体文献，建议通过PubMed或Web of Science检索关键词“recombinant recoverin”。
RCVRN (Recoverin) is a calcium-binding protein primarily expressed in retinal photoreceptor cells, where it plays a critical role in regulating phototransduction. Discovered in the 1990s, recoverin belongs to the neuronal calcium sensor (NCS) family and is characterized by its EF-hand motifs, which enable calcium-dependent interactions with target proteins. In darkness, elevated intracellular calcium levels promote recoverin’s binding to rhodopsin kinase (GRK1), inhibiting its activity and delaying the deactivation of photoexcited rhodopsin. During light adaptation, calcium concentrations drop, causing recoverin to dissociate from GRK1 and allowing rhodopsin inactivation to proceed, a mechanism essential for maintaining visual sensitivity across varying light conditions.
Recombinant RCVRN is produced using expression systems like *E. coli* or mammalian cell cultures, often tagged with histidine or other affinity markers for purification. Its recombinant form retains calcium-sensitive conformational changes and functional properties, making it a valuable tool for studying retinal signaling pathways. Researchers employ it to investigate diseases such as cancer-associated retinopathy (CAR), where autoantibodies against recoverin disrupt vision. Additionally, recombinant recoverin aids in structural studies, drug screening, and understanding calcium-mediated signaling crosstalk in neurodegeneration. Despite its physiological importance, aberrant recoverin expression or autoimmunity targeting this protein is linked to vision loss, underscoring its dual role as both a regulatory molecule and an autoantigen in pathology.
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