| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RDBP |
| Uniprot No | P18615 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-380aa |
| 氨基酸序列 | MLVIPPGLSE EEEALQKKFN KLKKKKKALL ALKKQSSSST TSQGGVKRSL SEQPVMDTAT ATEQAKQLVK SGAISAIKAE TKNSGFKRSR TLEGKLKDPE KGPVPTFQPF QRSISADDDL QESSRRPQRK SLYESFVSSS DRLRELGPDG EEAEGPGAGD GPPRSFDWGY EERSGAHSSA SPPRSRSRDR SHERNRDRDR DRERDRDRDR DRDRERDRDR DRDRDRDRER DRDRERDRDR DREGPFRRSD SFPERRAPRK GNTLYVYGED MTPTLLRGAF SPFGNIIDLS MDPPRNCAFV TYEKMESADQ AVAELNGTQV ESVQLKVNIA RKQPMLDAAT GKSVWGSLAV QNSPKGCHRD KRTQIVYSDD VYKENLVDGF |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RDBP(RNA-directed DNA Binding Protein)重组蛋白研究的虚构参考文献示例，供参考：
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1. **《Structural Analysis of RDBP and Its Role in Viral RNA Replication》**
*作者：Chen L. et al. (2020)*
**摘要**：本研究通过X射线晶体学解析了RDBP重组蛋白的三维结构，揭示了其与病毒RNA特异性结合的关键结构域，并验证了其在RNA病毒复制中的调控作用，为抗病毒药物设计提供了新靶点。
2. **《Optimization of RDBP Recombinant Expression in E. coli for Biomedical Applications》**
*作者：Wang Y. & Kumar S. (2018)*
**摘要**：文章报道了通过优化大肠杆菌表达系统的诱导条件和纯化工艺，高效制备高纯度RDBP重组蛋白的方法，并证明其在体外诊断试剂开发中的潜在应用价值。
3. **《RDBP-Mediated DNA Repair Mechanisms in Cancer Cells》**
*作者：Rodriguez J. et al. (2021)*
**摘要**：通过基因编辑技术敲除RDBP基因，发现其缺失导致癌细胞DNA损伤修复能力显著下降，提示RDBP重组蛋白可能作为癌症治疗的新生物标志物。
4. **《Functional Characterization of RDBP in Plant Genome Editing》**
*作者：Li H. & Park J. (2019)*
**摘要**：研究利用RDBP重组蛋白与CRISPR-Cas9系统结合，成功提高了植物基因编辑的靶向效率，为农业生物技术提供了改良工具。
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*注：以上文献为示例性内容，实际研究中请通过PubMed、Web of Science等数据库检索真实发表论文。*
**Background of RDBP Recombinant Protein**
The Recombinant DNA-Binding Protein (RDBP), also known as HIV-1 Rev or regulatory protein, is a critical viral factor in the life cycle of human immunodeficiency virus type 1 (HIV-1). It facilitates the nuclear export of unspliced or partially spliced viral RNA from the nucleus to the cytoplasm, enabling the production of structural proteins and viral genome packaging. RDBP contains an arginine-rich RNA-binding domain and a leucine-rich nuclear export signal, allowing it to shuttle between cellular compartments while interacting with specific secondary structures in viral RNA (e.g., the Rev Response Element, RRE).
Recombinant RDBP is engineered through molecular cloning and expressed in heterologous systems like *E. coli* or mammalian cells. Its production enables detailed study of HIV-1 replication mechanisms, particularly viral RNA trafficking and host-pathogen interactions. Researchers use it to investigate inhibitors targeting Rev-RRE binding, a potential therapeutic strategy to block viral replication. Beyond virology, RDBP serves as a model for studying protein-RNA interactions, nuclear transport, and the design of synthetic RNA-targeting tools.
The protein’s modular structure and functional versatility also make it relevant in gene therapy and mRNA delivery systems. Recombinant RDBP offers advantages such as high purity, scalability, and customizable modifications (e.g., tags for detection or purification), supporting both basic research and drug development efforts against HIV/AIDS.
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