| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RCAN3 |
| Uniprot No | Q9UKA8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 56-241aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSEAVFEAR EQKERFEALF TIYDDQVTFQ LFKSFRRVRI NFSKPEAAAR ARIELHETDF NGQKLKLYFA QVQMSGEVRD KSYLLPPQPV KQFLISPPAS PPVGWKQSED AMPVINYDLL CAVSKLGPGE KYELHAGTES TPSVVVHVCE SETEEEEETK NPKQKIAQTR RPDPPTAALN EPQTFDCAL |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RCAN3重组蛋白的3篇参考文献(信息基于公开研究整理，非真实文献)：
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1. **"Functional Characterization of RCAN3 Recombinant Protein in Calcineurin Signaling"**
*Smith et al.*
摘要：研究通过大肠杆菌表达系统纯化RCAN3重组蛋白，证明其能抑制钙调神经磷酸酶活性，并参与调控NFAT信号通路，提示其在神经和免疫系统中的潜在作用。
2. **"RCAN3 Recombinant Protein Attenuates Oxidative Stress in Cardiomyocytes"**
*Li & Wang*
摘要：利用哺乳动物细胞表达的RCAN3重组蛋白，发现其通过调节线粒体功能减少心肌细胞氧化应激损伤，为心血管疾病治疗提供新靶点。
3. **"Structural Insights into RCAN3 Isoforms via Recombinant Protein Crystallography"**
*Garcia-Rodriguez et al.*
摘要：通过X射线晶体学解析RCAN3重组蛋白结构，揭示不同剪接变体的构象差异，阐明其与钙调神经磷酸酶结合的分子机制。
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注：以上文献为示例性内容，实际研究中请通过学术数据库(如PubMed、Web of Science)检索真实发表论文。
**Background of RCAN3 Recombinant Protein**
RCAN3 (Regulator of Calcineurin 3) is a member of the RCAN family of proteins, which are evolutionarily conserved regulators of calcineurin, a calcium/calmodulin-dependent serine/threonine phosphatase. Calcineurin plays a critical role in numerous cellular processes, including immune response, cardiac hypertrophy, and neuronal development, by dephosphorylating substrates such as NFAT (Nuclear Factor of Activated T-cells). RCAN proteins, including RCAN1. RCAN2. and RCAN3. modulate calcineurin activity through competitive inhibition or scaffold-mediated regulation, thereby fine-tuning signaling pathways.
RCAN3. encoded by the *RCAN3* gene located on human chromosome 1. is the least characterized member of the family. It shares structural homology with RCAN1 and RCAN2. featuring conserved calcineurin-binding domains, but exhibits distinct tissue-specific expression patterns. RCAN3 is broadly expressed in the brain, heart, and skeletal muscle, suggesting roles in neurodevelopment and muscle function. Studies indicate that RCAN3 may act as a dual-function regulator, either suppressing or enhancing calcineurin activity depending on cellular context, though its precise mechanisms remain under investigation.
Recombinant RCAN3 protein is engineered for in vitro and in vivo studies to elucidate its biological functions. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), the recombinant protein retains functional domains necessary for interacting with calcineurin and other partners. Its applications include probing calcineurin-NFAT signaling dynamics, studying pathologies linked to calcineurin dysregulation (e.g., Down syndrome, cardiovascular diseases), and screening therapeutic agents targeting this pathway.
Despite progress, RCAN3's physiological relevance and disease associations remain less defined compared to RCAN1. which is strongly implicated in Down syndrome and Alzheimer’s disease. Ongoing research aims to clarify RCAN3's role in cellular homeostasis and its potential as a diagnostic or therapeutic target.
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