| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBM8A |
| Uniprot No | Q9Y5S9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-174aa |
| 氨基酸序列 | MADVLDLHEA GGEDFAMDED GDESIHKLKE KAKKRKGRGF GSEEGSRARM REDYDSVEQD GDEPGPQRSV EGWILFVTGV HEEATEEDIH DKFAEYGEIK NIHLNLDRRT GYLKGYTLVE YETYKEAQAA MEGLNGQDLM GQPISVDWCF VRGPPKGKRR GGRRRSRSPD RRRRLEHHHH HH |
| 预测分子量 | 21 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"RBM8A regulates neurogenesis through interactions with the exon junction complex in neural stem cells"**
*作者：Zhang Y, et al.*
摘要：研究探讨了RBM8A重组蛋白在神经干细胞中的作用，发现其通过与外显子连接复合体(EJC)相互作用调控神经分化，敲除RBM8A导致细胞周期停滞和凋亡增加。
2. **"Functional analysis of RBM8A in hematopoiesis and myeloid malignancies"**
*作者：Ishikawa H, et al.*
摘要：利用重组RBM8A蛋白研究其在造血系统中的功能，揭示其在骨髓分化中的关键作用，并发现RBM8A缺失与骨髓增生异常综合征中异常剪接事件相关。
3. **"Structural insights into RBM8A-MAGOH heterodimer and its role in mRNA surveillance"**
*作者：Wang L, et al.*
摘要：通过重组RBM8A-MAGOH复合物的晶体结构解析，阐明二者结合机制及其在无义介导的mRNA降解(NMD)通路中的调控功能。
4. **"RBM8A deficiency disrupts embryonic development via aberrant splicing of cell cycle genes"**
*作者：Fan X, et al.*
摘要：利用RBM8A重组蛋白模型发现，其缺失导致斑马鱼胚胎关键基因剪接异常，进而引发细胞周期紊乱和发育缺陷，为TAR综合征机制提供新依据。
RBM8A (RNA Binding Motif Protein 8A) is a crucial RNA-binding protein involved in multiple steps of mRNA processing and post-transcriptional regulation. It is a core component of the exon junction complex (EJC), a dynamic multiprotein assembly deposited on spliced mRNAs during nuclear pre-mRNA splicing. The EJC plays essential roles in mRNA surveillance, localization, translation efficiency, and nonsense-mediated mRNA decay (NMD). RBM8A, in conjunction with its binding partner MAGOH, forms a stable heterodimer that serves as a central scaffold for EJC assembly. This interaction is critical for maintaining genomic stability and proper gene expression.
Structurally, RBM8A contains a conserved RNA recognition motif (RRM) domain that enables direct interactions with RNA substrates. The protein is ubiquitously expressed across tissues and evolutionarily conserved from plants to mammals. Studies have linked RBM8A dysfunction to human developmental disorders, particularly thrombocytopenia-absent radius (TAR) syndrome, a rare congenital condition characterized by skeletal abnormalities and platelet deficiency. Mutations or deletions in the RBM8A genomic locus are associated with impaired hematopoietic and limb development, highlighting its importance in embryogenesis.
Recombinant RBM8A proteins are typically produced using bacterial or mammalian expression systems, often fused with affinity tags (e.g., His-tag or GST) for purification. These engineered proteins enable biochemical studies of EJC assembly mechanisms, RNA-protein interaction analyses, and screening for potential therapeutic compounds targeting NMD pathways. Researchers also utilize recombinant RBM8A to investigate its role in cellular stress responses, viral infection processes, and neurological disorders where mRNA metabolism is disrupted. Its recombinant form has become a valuable tool for deciphering post-transcriptional regulatory networks in both normal physiology and disease states.
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