| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBM11 |
| Uniprot No | P57052 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-281aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMFPAQEEADRTVFVGNLEARVREEILY ELFLQAGPLTKVTICKDREGKPKSFGFVCFKHPESVSYAIALLNGIRLYG RPINVQYRFGSSRSSEPANQSFESCVKINSHNYRNEEMLVGRSSFPMQYF PINNTSLPQEYFLFQKMQWHVYNPVLQLPYYEMTAPLPNSASVSSSLNHV PDLEAGPSSYKWTHQQPSDSDLYQMTAPLPNSASVSSSLNHVPDLEAGPS SYKWTHQQPSDSDLYQMNKRKRQKQTSDSDSSTDNNRGNECSQKFRKSKK KKRY |
| 预测分子量 | 35 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RBM11重组蛋白的模拟参考文献示例，涵盖其表达、功能、结构及疾病关联研究：
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1. **文献名称**：*Expression and Purification of Recombinant RBM11 for In Vitro RNA-Binding Assays*
**作者**：J. Smith et al.
**摘要**：本研究报道了在大肠杆菌系统中高效表达带His标签的重组RBM11蛋白，并通过Ni-NTA层析和尺寸排阻色谱纯化。实验验证了重组蛋白的RNA结合活性，证实其优先结合富含GU的RNA序列，为后续功能研究提供基础工具。
2. **文献名称**：*Functional Role of RBM11 in Alternative Splicing Regulation*
**作者**：L. Zhang et al.
**摘要**：通过siRNA敲低RBM11并结合重组蛋白回补实验，发现RBM11调控多个前体mRNA的选择性剪接，影响细胞周期相关基因的表达。重组RBM11恢复了剪接因子复合体的功能，提示其作为剪接调控因子的作用。
3. **文献名称**：*Crystal Structure of Recombinant RBM11 Reveals RNA Recognition Motif Architecture*
**作者**：M. Tanaka et al.
**摘要**：利用X射线晶体学解析了重组RBM11的RNA结合结构域(RRM)三维结构，揭示了其保守的β-α-β折叠及关键氨基酸残基。结构分析为理解RBM11与RNA及其他剪接蛋白的相互作用提供了分子基础。
4. **文献名称**：*RBM11 Overexpression in Cancer: Recombinant Protein-Based Mechanistic Insights*
**作者**：R. Patel et al.
**摘要**：研究发现RBM11在多种肿瘤中异常高表达。通过重组蛋白体外实验，证实其通过促进促增殖基因的剪接变体生成，加速癌细胞生长，为靶向RBM11的抗癌策略提供依据。
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这些示例反映了RBM11重组蛋白在表达技术、功能机制、结构解析及疾病相关性方面的研究，内容涵盖基础到应用层面。
RBM11 (RNA-Binding Motif Protein 11) is a member of the RNA-binding protein family, characterized by its conserved RNA recognition motifs (RRMs) that enable interactions with RNA molecules. It plays a role in post-transcriptional gene regulation, including pre-mRNA splicing, RNA stability, and translation control. Structurally, RBM11 contains an N-terminal RRM domain and a C-terminal region with potential zinc finger motifs, suggesting versatility in RNA binding and protein interactions. Its expression is detected in various tissues, with higher levels in the brain, testis, and certain cancer cells, implicating roles in development and disease.
Research indicates that RBM11 may regulate alternative splicing events, influencing the diversity of mRNA isoforms. For example, it has been linked to splicing modulation of genes involved in apoptosis and cell cycle control. Dysregulation of RBM11 is associated with cancers, including neuroblastoma and lung adenocarcinoma, where altered splicing patterns may drive tumor progression. In neurodevelopmental contexts, RBM11 interacts with other splicing factors, such as Sam68. to modulate neuronal mRNA processing, highlighting its importance in cellular differentiation.
Recombinant RBM11 protein is typically produced using expression systems like *E. coli* or mammalian cells, enabling functional studies *in vitro*. Purified RBM11 allows researchers to analyze its RNA-binding specificity, enzymatic activity, or interactions with spliceosome components. It also serves as a tool for investigating molecular mechanisms underlying splicing defects in diseases. Despite progress, RBM11’s full functional repertoire remains unclear, necessitating further exploration of its regulatory networks and therapeutic potential. Recombinant protein studies continue to bridge gaps in understanding its role in RNA biology and pathology.
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