| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBBP4 |
| Uniprot No | Q09028 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-425aa |
| 氨基酸序列 | ADKEAAFDD AVEERVINEE YKIWKKNTPF LYDLVMTHAL EWPSLTAQWL PDVTRPEGKD FSIHRLVLGT HTSDEQNHLV IASVQLPNDD AQFDASHYDS EKGEFGGFGS VSGKIEIEIK INHEGEVNRA RYMPQNPCII ATKTPSSDVL VFDYTKHPSK PDPSGECNPD LRLRGHQKEG YGLSWNPNLS GHLLSASDDH TICLWDISAV PKEGKVVDAK TIFTGHTAVV EDVSWHLLHE SLFGSVADDQ KLMIWDTRSN NTSKPSHSVD AHTAEVNCLS FNPYSEFILA TGSADKTVAL WDLRNLKLKL HSFESHKDEI FQVQWSPHNE TILASSGTDR RLNVWDLSKI GEEQSPEDAE DGPPELLFIH GGHTAKISDF SWNPNEPWVI CSVSEDNIMQ VWQMAENIYN DEDPEGSVDP EGQGS |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于RBBP4重组蛋白的参考文献及其摘要概括：
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1. **文献名称**: "Structural insights into the role of RBBP4 in chromatin remodeling complexes"
**作者**: Low, J.K., et al.
**摘要**: 本研究通过重组表达人源RBBP4蛋白，解析其与组蛋白H3-H4四聚体的结合机制，发现RBBP4通过N端结构域稳定组蛋白，促进染色质重塑复合体(如NuRD)的组装，为表观遗传调控提供结构基础。
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2. **文献名称**: "Recombinant RBBP4 as a potential therapeutic target in breast cancer"
**作者**: Zhang, Y., et al.
**摘要**: 利用重组RBBP4蛋白进行体外功能实验，发现其过表达通过调控细胞周期蛋白(如Cyclin D1)促进乳腺癌细胞增殖。抑制RBBP4可显著降低肿瘤生长，提示其作为癌症治疗靶点的潜力。
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3. **文献名称**: "Biochemical characterization of RBBP4 interaction with histone deacetylases"
**作者**: Chen, L., et al.
**摘要**: 通过重组RBBP4与HDAC1/2的共纯化实验，揭示RBBP4作为支架蛋白介导HDAC与染色质的结合，并证明其乙酰化修饰可动态调控复合体活性，影响基因沉默过程。
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**备注**：以上文献为示例，实际引用时需根据具体研究内容核对真实文献信息。如需精确文献，建议通过PubMed或Web of Science检索关键词“RBBP4 recombinant”、“RbAp48 purification”等获取。
**Background of RBBP4 Recombinant Protein**
RBBP4 (Retinoblastoma-Binding Protein 4), also known as RbAp48. is a highly conserved histone chaperone and a core component of chromatin-modifying complexes. It belongs to the WD40 repeat protein family, characterized by propeller-like domains that mediate protein-protein interactions. RBBP4 plays a pivotal role in epigenetic regulation by associating with histone deacetylases (HDACs) in complexes like Sin3. NuRD, and Polycomb repressive complexes (PRC2), facilitating chromatin remodeling and gene expression control. It is essential for processes such as DNA replication, repair, cell cycle progression, and differentiation.
Recombinant RBBP4 is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to yield a purified, biologically active form of the protein. Its recombinant version retains the ability to bind histones (particularly H3-H4 tetramers) and interact with partner proteins, making it a valuable tool for studying chromatin dynamics, histone modification mechanisms, and the structural basis of epigenetic regulation. Researchers employ it in *in vitro* assays, structural studies (e.g., X-ray crystallography), and functional analyses to dissect its role in diseases linked to epigenetic dysregulation, such as cancer, neurodegenerative disorders, and developmental abnormalities.
The recombinant protein’s availability has advanced drug discovery efforts, enabling high-throughput screening for inhibitors targeting RBBP4-containing complexes. Challenges include maintaining proper folding and post-translational modifications, often addressed by optimizing expression systems. Overall, RBBP4 recombinant protein serves as a critical reagent for unraveling the molecular interplay between chromatin structure, gene regulation, and disease pathogenesis.
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