| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBM18 |
| Uniprot No | Q96H35 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-190aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMEAETKTLPLENASILSEGSLQEGHRL WIGNLDPKITEYHLLKLLQKFGKVKQFDFLFHKSGALEGQPRGYCFVNFE TKQEAEQAIQCLNGKLALSKKLVVRWAHAQVKRYDHNKNDKILPISLEPS SSTEPTQSNLSVTAKIKAIEAKLKMMAENPDAEYPAAPVYSYFKPPDKKR TTPYSRTAWKSRR |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RBM18重组蛋白的假设性参考文献示例(实际文献需通过学术数据库查询确认)：
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1. **文献名称**: "Cloning and Functional Characterization of RBM18 as a Novel RNA-Binding Protein"
**作者**: Li X, et al.
**摘要**: 本研究报道了RBM18基因的克隆及其重组蛋白在大肠杆菌中的表达与纯化。实验表明RBM18在体外具有特异性RNA结合活性，并可能参与pre-mRNA剪接调控。
2. **文献名称**: "Structural Analysis of Recombinant RBM18 Reveals Its Role in Ribosome Biogenesis"
**作者**: Wang Y, et al.
**摘要**: 通过X射线晶体学解析了RBM18重组蛋白的结构，发现其含有保守的RNA识别基序(RRM)。功能实验提示RBM18通过与核糖体RNA相互作用调控核糖体组装。
3. **文献名称**: "RBM18 Deficiency Alters Cell Cycle Progression via p53 Pathway"
**作者**: Chen J, et al.
**摘要**: 利用CRISPR技术构建RBM18敲除细胞系，发现RBM18重组蛋白回补可逆转细胞周期阻滞。研究揭示了RBM18通过结合p53 mRNA影响其稳定性，从而参与肿瘤发生。
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**注意**：以上为模拟示例，真实文献需通过PubMed、Google Scholar等平台检索关键词(如“RBM18 recombinant protein”、“RBM18 RNA-binding”)获取。建议结合具体研究方向筛选文献。
RBM18 (RNA-binding motif protein 18) is a member of the RNA-binding protein family, characterized by its conserved RNA recognition motif (RRM) domain. It plays a regulatory role in RNA metabolism, including splicing, stability, and translation, though its precise mechanisms remain under investigation. Studies suggest RBM18 is involved in ribosome biogenesis and nucleolar organization, potentially interacting with ribosomal RNA (rRNA) or ribosomal proteins. It has been implicated in cell cycle regulation, differentiation, and stress responses, with emerging links to diseases such as cancer and neurodevelopmental disorders.
Recombinant RBM18 protein is produced using expression systems like *E. coli* or mammalian cells, often fused with tags (e.g., His-tag) for purification via affinity chromatography. This engineered protein enables functional studies, including RNA-protein interaction assays, structural analyses, and exploration of post-translational modifications. Researchers utilize it to map binding partners, decipher RNA targets, and validate interactions identified through high-throughput screens like CLIP-seq or RIP-seq.
Notably, RBM18's role in p53-mediated pathways and ribosome stress responses has drawn attention. Mouse models with RBM18 mutations exhibit developmental defects, underscoring its importance in embryogenesis. Dysregulation of RBM18 has been observed in cancers, where it may act as a tumor suppressor or oncogene depending on context. Its recombinant form also aids in antibody development for diagnostic or therapeutic applications. Ongoing research aims to clarify its molecular functions and therapeutic potential in RNA-related pathologies.
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