| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBP7 |
| Uniprot No | Q96R05 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-134aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MPADLSGTWT LLSSDNFEGY MLALGIDFAT RKIAKLLKPQ KVIEQNGDSF TIHTNSSLRN YFVKFKVGEE FDEDNRGLDN RKCKSLVIWD NDRLTCIQKG EKKNRGWTHW IEGDKLHLEM FCEGQVCKQT FQRA |
| 预测分子量 | 18 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RBP7重组蛋白的3篇代表性文献(注：文献内容基于真实研究概括，但作者及期刊信息为虚拟示例)：
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1. **文献名称**: *"Recombinant human RBP7 expression in E. coli and its role in cellular retinol transport"*
**作者**: Li, X. et al.
**摘要**: 本研究通过大肠杆菌系统成功表达并纯化重组人源RBP7蛋白，验证其与视黄醇结合的能力。实验表明，RBP7在肝细胞中可能通过调控视黄醇的胞内转运参与维生素A代谢通路。
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2. **文献名称**: *"Structural characterization of RBP7 and its interaction with STRA6 receptor"*
**作者**: Martinez, R. & Kumar, S.
**摘要**: 利用X射线晶体学解析了重组RBP7的三维结构，并发现其与细胞表面受体STRA6存在特异性相互作用，揭示了RBP7在视黄酸信号传导中的潜在分子机制。
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3. **文献名称**: *"RBP7 overexpression promotes tumor angiogenesis in breast cancer models"*
**作者**: Chen, Y. et al.
**摘要**: 通过重组RBP7蛋白处理乳腺癌细胞，发现其显著上调血管内皮生长因子(VEGF)的表达，提示RBP7可能通过促血管生成途径参与肿瘤微环境调控。
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**备注**：以上文献标题及结论为示例性质，实际研究中建议通过PubMed或Web of Science以“RBP7 recombinant protein”或“Retinol-Binding Protein 7”为关键词检索最新文献。
**Background of RBP7 Recombinant Protein**
Retinol-binding protein 7 (RBP7), a member of the retinol-binding protein (RBP) family, plays a critical role in retinoid signaling and lipid-soluble vitamin transport. Unlike its well-studied homolog RBP4. which is primarily involved in systemic retinol (vitamin A) transport, RBP7 is broadly expressed in tissues such as adipose, liver, and brain, suggesting distinct physiological functions. It binds to retinol and facilitates its intracellular trafficking, influencing cellular processes like differentiation, metabolism, and gene regulation.
RBP7 has garnered attention for its potential links to metabolic and inflammatory diseases. Studies indicate its dysregulation in obesity, insulin resistance, and certain cancers, implicating it in metabolic homeostasis and disease progression. For instance, RBP7 may modulate lipid metabolism in adipocytes or regulate inflammatory responses in macrophages, though its precise mechanisms remain under investigation.
Recombinant RBP7 is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to ensure high purity and bioactivity. This engineered protein retains the ability to bind retinoids and interact with cellular receptors, making it a valuable tool for *in vitro* and *in vivo* studies. Researchers utilize it to dissect RBP7’s role in retinoid signaling pathways, explore its therapeutic potential in metabolic disorders, or develop diagnostic assays targeting vitamin A-related pathologies.
Despite progress, gaps persist in understanding RBP7’s tissue-specific functions and its interplay with other RBPs. Ongoing research aims to clarify its contributions to health and disease, positioning recombinant RBP7 as a pivotal reagent in both basic and translational studies.
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