| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBP2 |
| Uniprot No | P50120 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-134aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMTRDQN GTWEMESNEN FEGYMKALDI DFATRKIAVR LTQTKVIDQD GDNFKTKTTS TFRNYDVDFT VGVEFDEYTK SLDNRHVKAL VTWEGDVLVC VQKGEKENRG WKQWIEGDKL YLELTCGDQV CRQVFKKK |
| 预测分子量 | 18 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RBP2重组蛋白的参考文献，按研究主题分类列举：
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1. **标题**：*Expression and Purification of Recombinant Human Retinol-Binding Protein 2 (RBP2) in Escherichia coli*
**作者**：Smith J, et al.
**摘要**：研究报道了在大肠杆菌中高效表达重组人RBP2的优化方法，通过亲和层析纯化获得高纯度蛋白，并通过质谱和圆二色性光谱验证其结构与天然蛋白的一致性。
2. **标题**：*Structural Characterization of Recombinant RBP2 and Its Interaction with Retinol*
**作者**：Lee H, et al.
**摘要**：利用X射线晶体学解析了重组RBP2的三维结构，揭示了其与视黄醇结合的分子机制，关键疏水残基在稳定配体结合中起重要作用。
3. **标题**：*Functional Analysis of Recombinant RBP2 in Vitamin A-Deficient Mice*
**作者**：Garcia R, et al.
**摘要**：通过动物实验证实，注射重组RBP2可显著改善维生素A缺乏小鼠的视黄醇转运能力，恢复视网膜功能，表明其潜在治疗价值。
4. **标题**：*Development of a Recombinant RBP2-Based ELISA for Vitamin A Status Assessment*
**作者**：Chen L, et al.
**摘要**：开发了一种基于重组RBP2的ELISA检测法，用于快速评估人体维生素A水平，临床样本验证显示与传统方法高度一致，适合资源有限地区应用。
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**注**：以上文献为示例，实际引用时请核实具体来源及发表信息。若需扩展，建议在PubMed或Web of Science中以关键词“recombinant RBP2”、“Retinol-Binding Protein 2 expression”进一步检索。
**Background of RBP2 Recombinant Protein**
Recombinant Retinol-Binding Protein 2 (RBP2), also known as cellular retinol-binding protein 2 (CRBP2), is a small intracellular protein critical for vitamin A (retinol) metabolism. It belongs to the lipocalin family and is primarily expressed in the small intestine, where it facilitates the absorption, transport, and storage of dietary retinol. RBP2 binds retinol with high specificity, protecting it from oxidation and ensuring its efficient delivery to enzymes like lecithin-retinol acyltransferase (LRAT) for esterification. This process is essential for maintaining vision, immune function, and cellular differentiation.
The production of recombinant RBP2 leverages genetic engineering techniques. The RBP2 gene is cloned into expression vectors and expressed in systems such as *E. coli* or mammalian cells. Purification methods like affinity chromatography yield high-purity RBP2. often tagged with markers (e.g., His-tag) for ease of isolation. Recombinant RBP2 retains native structural and functional properties, making it invaluable for biochemical studies, including ligand-binding assays, structural analysis (e.g., X-ray crystallography), and investigations into retinol-mediated signaling pathways.
Research on RBP2 has expanded beyond its metabolic role. Dysregulation of RBP2 is linked to pathologies such as intestinal malabsorption syndromes and certain cancers. Its recombinant form is also explored for therapeutic applications, such as drug delivery systems targeting vitamin A-dependent processes. Additionally, RBP2 serves as a biomarker in nutritional studies to assess vitamin A status. Overall, recombinant RBP2 is a versatile tool bridging basic research and clinical innovation in nutrition and disease biology.
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