| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBM3 |
| Uniprot No | P98179 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-157aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMSSEEGKLFVGGLNFNTDEQALEDHFS SFGPISEVVVVKDRETQRSRGFGFITFTNPEHASVAMRAMNGESLDGRQI RVDHAGKSARGTRGGGFGAHGRGRSYSRGGGDQGYGSGRYYDSRPGGYGY GYGRSRDYNGRNQGGYDRYSGGNYRDNYDN |
| 预测分子量 | 19 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RBM3重组蛋白的3篇代表性文献示例(注：文献信息为模拟示例，建议通过学术数据库核实具体内容)：
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1. **文献名称**: *"Cold-inducible RBM3 mediates adaptive neuroprotection through translational regulation"*
**作者**: Pilotte, J., et al.
**摘要**: 研究揭示了RBM3在低温应激下通过选择性翻译调控机制促进神经细胞存活的作用，重组RBM3蛋白在体外模型中表现出抑制细胞凋亡的能力。
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2. **文献名称**: *"RNA-binding motif protein 3 interacts with the translation machinery and enhances protein synthesis under hypothermia"*
**作者**: Dresios, S., et al.
**摘要**: 本文发现RBM3重组蛋白通过与核糖体结合，在低温条件下维持蛋白质翻译效率，为低温治疗中的细胞保护机制提供了分子依据。
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3. **文献名称**: *"RBM3 overexpression correlates with cancer progression and therapeutic resistance"*
**作者**: Zhuang, X., et al.
**摘要**: 研究利用重组RBM3蛋白验证其在肿瘤细胞中的促增殖功能，发现其通过调控特定mRNA稳定性增强癌细胞对化疗的抵抗性。
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**提示**：建议通过PubMed或Google Scholar搜索关键词“RBM3 recombinant protein”获取最新文献，并关注其与低温适应、癌症、神经退行性疾病相关的功能研究。
**Background of RBM3 Recombinant Protein**
RNA-binding motif protein 3 (RBM3) is a cold-inducible RNA-binding protein belonging to the cold shock protein (CSP) family, initially identified for its rapid upregulation under hypothermic conditions. It plays a critical role in cellular stress responses, particularly in promoting cell survival during low-temperature stress. Structurally, RBM3 contains an N-terminal RNA recognition motif (RRM) that facilitates interactions with target mRNAs, influencing their stability, translation, or splicing.
Recombinant RBM3 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, biologically active forms for research and therapeutic applications. Its recombinant production enables studies on mechanisms underlying cold adaptation, neuroprotection, and cancer biology. Notably, RBM3 demonstrates neuroprotective effects by suppressing apoptosis, enhancing synaptic plasticity, and maintaining protein synthesis under stress, making it a potential therapeutic candidate for neurodegenerative diseases like Alzheimer’s and Parkinson’s.
In oncology, RBM3 exhibits dual roles—acting as both a tumor suppressor and promoter depending on cancer type. It regulates proliferation, metastasis, and chemoresistance by modulating oncogenic signaling pathways (e.g., p53. HIF-1α). Additionally, its cold-inducible nature has spurred interest in hypothermia-based therapies and organ preservation strategies.
Despite its promise, challenges remain in understanding tissue-specific functions, optimal delivery methods, and long-term safety. Recombinant RBM3 continues to be a valuable tool for unraveling stress-responsive pathways and developing interventions for neurodegeneration, cancer, and ischemia-reperfusion injuries.
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