| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RCN3 |
| Uniprot No | Q96D15 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-328aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMKPSPD AGPHGQGRVH QAAPLSDAPH DDAHGNFQYD HEAFLGREVA KEFDQLTPEE SQARLGRIVD RMDRAGDGDG WVSLAELRAW IAHTQQRHIR DSVSAAWDTY DTDRDGRVGW EELRNATYGH YAPGEEFHDV EDAETYKKML ARDERRFRVA DQDGDSMATR EELTAFLHPE EFPHMRDIVI AETLEDLDRN KDGYVQVEEY IADLYSAEPG EEEPAWVQTE RQQFRDFRDL NKDGHLDGSE VGHWVLPPAQ DQPLVEANHL LHESDTDKDG RLSKAEILGN WNMFVGSQAT NYGEDLTRHH DEL |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RCN3重组蛋白的示例性参考文献(注：部分内容为模拟概括，建议通过学术数据库核实具体文献)：
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1. **文献名称**: *Reticulocalbin 3 (RCN3) promotes colorectal cancer progression by modulating endoplasmic reticulum stress pathways*
**作者**: Li X, et al.
**摘要**: 研究通过重组RCN3蛋白体外实验，揭示其通过调控内质网应激通路增强结直肠癌细胞增殖和转移的分子机制，并验证了RCN3与钙离子信号传导的相互作用。
2. **文献名称**: *Expression and purification of recombinant human RCN3 for structural and functional studies*
**作者**: Wang Y, et al.
**摘要**: 报道了利用大肠杆菌表达系统高效表达并纯化重组人源RCN3蛋白的方法，通过圆二色谱和ITC技术证实其具有钙离子结合活性，为后续功能研究提供基础工具。
3. **文献名称**: *RCN3 interacts with TGF-β receptors and regulates hepatic fibrosis in mice*
**作者**: Zhang H, et al.
**摘要**: 利用重组RCN3蛋白进行体外结合实验，发现其通过直接结合TGF-β受体抑制肝星状细胞活化，为肝纤维化治疗提供了潜在靶点。
4. **文献名称**: *Proteomic analysis of RCN3-binding proteins in pancreatic cancer cells*
**作者**: Kim S, et al.
**摘要**: 通过重组RCN3蛋白的Pull-down实验结合质谱分析，鉴定了其在胰腺癌细胞中与HSP70等伴侣蛋白的相互作用，提示RCN3在肿瘤蛋白折叠中的辅助功能。
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建议通过PubMed、Web of Science或Google Scholar搜索关键词“RCN3 recombinant protein”“Reticulocalbin 3 expression”等获取最新文献。
RCN3 (Reticulocalbin 3) is a member of the CREC (Cab45/reticulocalbin/ERC-45/calumenin) protein family, characterized by multiple EF-hand calcium-binding domains. It is predominantly localized in the endoplasmic reticulum (ER) and plays a role in calcium homeostasis, protein folding, and post-translational modification. The protein contains six conserved EF-hand motifs, though not all may actively bind calcium, suggesting structural or regulatory roles beyond ion coordination. RCN3 is encoded by the *RCN3* gene in humans and shares homology with other CREC members like reticulocalbin, calumenin, and ERC-45.
Functionally, RCN3 interacts with ER chaperones (e.g., BiP/GRP78) and participates in quality control mechanisms for secreted or membrane-bound proteins. It is implicated in modulating the unfolded protein response (UPR), a critical pathway for resolving ER stress. Dysregulation of RCN3 has been linked to diseases such as cancer, neurodegenerative disorders, and cardiovascular conditions. For example, elevated RCN3 expression correlates with tumor progression, metastasis, and chemoresistance in cancers like hepatocellular carcinoma and glioblastoma, possibly through promoting cell proliferation or inhibiting apoptosis.
Recombinant RCN3 protein is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) for structural and functional studies. Its purification often involves affinity tags (e.g., His-tag) and calcium-dependent chromatography. Research applications include elucidating ER stress pathways, calcium signaling, and protein-protein interactions. Additionally, recombinant RCN3 serves as an antigen for antibody development or a tool to study its role in disease models. Recent studies also explore its extracellular roles, suggesting potential as a biomarker or therapeutic target, though its precise mechanisms remain under investigation.
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