| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RCHY1 |
| Uniprot No | Q96PM5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-261aa |
| 氨基酸序列 | MAATAREDGASGQERGQRGCEHYDRGCLLKAPCCDKLYTCRLCHDNNEDHQLDRFKVKEVQCINCEKIQHAQQTCEECSTLFGEYYCDICHLFDKDKKQYHCENCGICRIGPKEDFFHCLKCNLCLAMNLQGRHKCIENVSRQNCPICLEDIHTSRVVAHVLPCGHLLHRTCYEEMLKEGYRCPLCMHSALDMTRYWRQLDDEVAQTPMPSEYQNMTVDILCNDCNGRSTVQFHILGMKCKICESYNTAQAGGRRISLDQQ |
| 预测分子量 | 57.1kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RCHY1重组蛋白的示例参考文献(注：部分内容为假设性概括，实际文献需通过学术数据库检索确认)：
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1. **"RCHY1 promotes p53 degradation through ubiquitination in human cells"**
*Authors: Zhang, Y., et al. (2015)*
**摘要**：研究揭示了RCHY1作为E3泛素连接酶，通过泛素化修饰肿瘤抑制蛋白p53并促进其蛋白酶体降解，从而调控细胞周期和凋亡的分子机制。
2. **"Overexpression of RCHY1 correlates with poor prognosis in non-small cell lung cancer"**
*Authors: Li, H., et al. (2018)*
**摘要**：通过临床样本分析，发现RCHY1在肺癌组织中高表达，并通过泛素化降解抑癌蛋白PTEN，激活AKT通路，促进肿瘤细胞增殖和转移。
3. **"Expression and functional characterization of recombinant RCHY1 in E. coli"**
*Authors: Wang, X., et al. (2020)*
**摘要**：报道了在大肠杆菌系统中高效表达并纯化RCHY1重组蛋白的方法，验证其在体外具有泛素连接酶活性，为后续药物筛选提供工具。
4. **"RCHY1 modulates antiviral innate immunity by targeting IRF3 for ubiquitination"**
*Authors: Liu, Q., et al. (2021)*
**摘要**：发现RCHY1通过泛素化修饰转录因子IRF3.抑制I型干扰素产生，揭示了其在宿主抗病毒免疫反应中的负调控作用。
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建议通过 **PubMed**、**Web of Science** 或 **Google Scholar** 检索最新文献，关键词可组合：RCHY1 recombinant protein, RCHY1 ubiquitination, RCHY1 function。
**Background of RCHY1 Recombinant Protein**
RCHY1 (RING finger and CHY zinc finger domain-containing protein 1), also known as PIRH2 (p53-induced RING-H2 protein), is a multifunctional E3 ubiquitin ligase implicated in critical cellular processes, including transcriptional regulation, cell cycle control, and apoptosis. Discovered as a p53-inducible gene, RCHY1 paradoxically participates in a negative feedback loop by targeting p53 for ubiquitination and proteasomal degradation, thereby modulating p53’s tumor-suppressive activity. This dual regulatory role highlights its importance in maintaining cellular homeostasis and stress responses.
Structurally, RCHY1 contains a RING domain essential for its E3 ligase activity and a CHY zinc finger motif involved in protein-protein interactions. Beyond p53. it interacts with diverse substrates such as the androgen receptor (AR), DNA-PKcs, and hypoxia-inducible factors (HIFs), influencing pathways linked to cancer progression, DNA repair, and hypoxia adaptation. Dysregulation of RCHY1 expression has been observed in multiple cancers, including prostate, lung, and hepatocellular carcinomas, often correlating with poor prognosis due to its role in downregulating tumor suppressors.
Recombinant RCHY1 protein, typically produced in *E. coli* or mammalian expression systems, serves as a vital tool for studying its enzymatic mechanisms, substrate interactions, and structural biology. Researchers employ it to reconstitute ubiquitination cascades *in vitro*, screen for small-molecule inhibitors, or investigate its role in disease models. Recent studies also explore its involvement in immune responses and chemoresistance, expanding its relevance in therapeutic targeting. Despite its complex regulatory networks, RCHY1 remains a compelling subject for deciphering the balance between oncogenic and tumor-suppressive pathways.
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