| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RERG |
| Uniprot No | Q96A58 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-199aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MAKSAEVKLA IFGRAGVGKS ALVVRFLTKR FIWEYDPTLE STYRHQATID DEVVSMEILD TAGQEDTIQR EGHMRWGEGF VLVYDITDRG SFEEVLPLKN ILDEIKKPKN VTLILVGNKA DLDHSRQVST EEGEKLATEL ACAFYECSAC TGEGNITEIF YELCREVRRR RMVQGKTRRR SSTTHVKQAI NKMLTKISS |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RERG重组蛋白的3篇示例参考文献(注：内容为模拟概括，具体文献需根据实际数据库检索)：
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1. **标题**：*"Expression and Purification of Recombinant RERG Protein in Escherichia coli"*
**作者**：Smith A, et al.
**摘要**：本研究成功构建了RERG基因的原核表达载体，并利用大肠杆菌系统高效表达可溶性RERG重组蛋白。通过亲和层析纯化获得高纯度蛋白，并验证其具有GTP酶活性，为后续功能研究奠定基础。
2. **标题**：*"RERG Recombinant Protein Suppresses Breast Cancer Cell Proliferation via ERK Pathway Inhibition"*
**作者**：Jones B, et al.
**摘要**：通过体外实验证明，纯化的RERG重组蛋白能够抑制乳腺癌细胞系的增殖，并降低ERK信号通路的磷酸化水平，提示其可能通过调控MAPK/ERK通路发挥肿瘤抑制作用。
3. **标题**：*"Structural Characterization of RERG Using Recombinant Protein and X-ray Crystallography"*
**作者**：Lee C, et al.
**摘要**：利用昆虫细胞表达系统制备RERG重组蛋白，通过X射线晶体学解析其三维结构，揭示其与Ras家族蛋白的结构差异及潜在的分子作用界面。
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如需准确文献，建议在PubMed或Web of Science中检索关键词“RERG recombinant protein”或“RERG protein purification”。
**Background of RERG Recombinant Protein**
The RERG (Ras-related and Estrogen-Regulated Growth inhibitor) protein is a member of the Ras superfamily of small GTPases, known for their roles in regulating cell proliferation, differentiation, and survival. Discovered in 2002. RERG was identified as an estrogen-responsive gene in breast cancer cells, distinguishing it from other Ras members due to its tumor-suppressive functions. Unlike oncogenic Ras proteins, RERG acts as a growth inhibitor by binding and hydrolyzing GTP, thereby modulating downstream signaling pathways such as the MAPK cascade. Its expression is tightly regulated by estrogen receptor (ER) signaling, linking it to hormone-responsive cancers, particularly breast cancer.
RERG is frequently downregulated or lost in malignancies, correlating with poor prognosis and tumor progression. Epigenetic silencing, such as promoter hypermethylation, and loss of heterozygosity are common mechanisms behind its reduced expression. Functional studies highlight its role in suppressing cell cycle progression, promoting apoptosis, and inhibiting metastasis. These characteristics position RERG as a critical regulator of cellular homeostasis and a potential biomarker or therapeutic target in cancer.
Recombinant RERG protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), enables *in vitro* studies to dissect its biochemical properties, GTPase activity, and interactions with signaling molecules. It also serves as a tool for drug screening to identify compounds that restore or mimic RERG function in cancer cells. Research on RERG recombinant protein continues to advance understanding of its molecular mechanisms and therapeutic potential in oncology.
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