| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | REXO1 |
| Uniprot No | Q8N1G1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1060-1221aa |
| 氨基酸序列 | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSIYAL DCEMSYTTYG LELTRVTVVD TDVHVVYDTF VKPDNEIVDY NTRFSGVTEA DLADTSVTLR DVQAVLLSMF SADTILIGHS LESDLLALKV IHSTVVDTSV LFPHRLGLPY KRSLRNLMAD YLRQIIQDNV DGHSSSEDAG ACMHLVIWKV REDAKTKR |
| 预测分子量 | 22 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于REXO1重组蛋白的3篇假设性参考文献示例，供参考：
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1. **文献名称**：*"Expression and Functional Characterization of Recombinant REXO1 in Eukaryotic Cells"*
**作者**：Smith, J. et al.
**摘要**：本研究利用哺乳动物表达系统成功制备重组REXO1蛋白，证实其具有RNA外切酶活性，并在体外实验中证明其参与核糖体RNA加工过程。
2. **文献名称**：*"Structural Insights into REXO1’s Role in Mitochondrial RNA Degradation"*
**作者**：Li, X. et al.
**摘要**：通过大肠杆菌表达重组REXO1蛋白并解析其晶体结构，揭示了其与底物RNA结合的活性位点，为线粒体RNA代谢异常相关疾病机制提供依据。
3. **文献名称**：*"REXO1 Knockdown and Recombinant Protein Rescue Experiments Reveal Its Essential Role in Embryonic Development"*
**作者**：Wang, Y. et al.
**摘要**：利用重组REXO1蛋白回补实验，验证其在胚胎干细胞分化中的关键作用，表明其通过调控特定mRNA稳定性影响发育通路。
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**备注**：以上文献为模拟内容，实际研究中建议通过PubMed或Web of Science检索真实论文(如搜索关键词“REXO1 recombinant”或“REXO1 purification”)。若需具体文献协助，可提供更详细的研究方向或背景信息。
**Background of REXO1 Recombinant Protein**
REXO1 (RNA exonuclease 1 homolog) is a conserved eukaryotic protein belonging to the exonuclease family, characterized by its role in RNA processing and degradation. It shares structural homology with the yeast Rex1p and Rex2p proteins, which are critical for ribosomal RNA (rRNA) maturation and small non-coding RNA quality control. REXO1 contains a conserved N-terminal exonuclease domain responsible for its enzymatic activity, enabling it to degrade RNA in a 3'-to-5' direction. This activity is essential for trimming precursor rRNA, removing aberrant RNAs, and maintaining cellular RNA homeostasis.
The recombinant form of REXO1 is produced through genetic engineering, typically expressed in bacterial or eukaryotic systems (e.g., *E. coli* or mammalian cells) to ensure proper folding and functionality. Recombinant REXO1 is widely used in biochemical studies to dissect its enzymatic mechanisms, substrate specificity, and interaction partners. Research has linked REXO1 dysfunction to human diseases, including ribosomopathies and cancers, where altered RNA processing contributes to pathological states.
Additionally, recombinant REXO1 serves as a tool for exploring RNA metabolism pathways and developing therapeutic strategies targeting RNA degradation processes. Its study also provides insights into evolutionary conservation of RNA quality control systems across species. Recent advances in structural biology utilizing recombinant REXO1 have revealed atomic-level details of its catalytic site, guiding drug design efforts. Overall, REXO1 recombinant protein is a vital resource for understanding RNA biology and its implications in health and disease.
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