| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RGN |
| Uniprot No | Q15493 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-299aa |
| 氨基酸序列 | MSSIKIECVL PENCRCGESP VWEEVSNSLL FVDIPAKKVC RWDSFTKQVQ RVTMDAPVSS VALRQSGGYV ATIGTKFCAL NWKEQSAVVL ATVDNDKKNN RFNDGKVDPA GRYFAGTMAE ETAPAVLERH QGALYSLFPD HHVKKYFDQV DISNGLDWSL DHKIFYYIDS LSYSVDAFDY DLQTGQISNR RSVYKLEKEE QIPDGMCIDA EGKLWVACYN GGRVIRLDPV TGKRLQTVKL PVDKTTSCCF GGKNYSEMYV TCARDGMDPE GLLRQPEAGG IFKITGLGVK GIAPYSYAG |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RGN(Regucalcin)重组蛋白的参考文献示例，包括文献名称、作者及摘要概述：
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1. **文献名称**: *"Expression and purification of recombinant regucalcin in Escherichia coli and its role in calcium signaling"*
**作者**: Yamaguchi M., Yamamoto T.
**摘要**: 本研究报道了在大肠杆菌中高效表达重组Regucalcin(RGN)的方法，并通过亲和层析纯化获得高纯度蛋白。功能实验表明，重组RGN通过调节细胞内钙离子浓度影响肝细胞凋亡和增殖信号通路。
2. **文献名称**: *"Regucalcin suppresses cell proliferation in human breast cancer via downregulation of oncogenic signaling pathways"*
**作者**: Sheikh B.N., Mori K., Jones C.
**摘要**: 通过哺乳动物表达系统制备重组RGN蛋白，研究发现其在乳腺癌细胞中通过抑制ERK和Akt信号通路显著降低肿瘤细胞增殖，提示RGN可能成为潜在的癌症治疗靶点。
3. **文献名称**: *"Recombinant regucalcin mitigates oxidative stress in a rat model of hepatic ischemia-reperfusion injury"*
**作者**: Li H., Zhang Y., Wang J.
**摘要**: 利用昆虫细胞表达系统生产重组RGN，并在大鼠肝缺血再灌注损伤模型中验证其功能。结果显示，重组RGN通过清除自由基和减少脂质过氧化改善肝组织损伤。
4. **文献名称**: *"Structural and functional characterization of regucalcin as a calcium-binding protein in neuronal cells"*
**作者**: Tanaka Y., Yamaguchi M.
**摘要**: 通过X射线晶体学解析重组RGN的三维结构，揭示其钙离子结合位点的分子机制。体外实验证实RGN对神经元钙稳态具有调控作用，可能参与神经退行性疾病的病理过程。
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注：上述文献为示例性内容，实际引用时需核实真实来源及详细信息。建议通过PubMed或Web of Science以关键词“recombinant regucalcin”或“RGN recombinant protein”检索最新研究。
**Background of RGN Recombinant Protein**
Recombinant RGN (Regnase-1), also known as ZC3H12A or MCPIP1. is a ribonuclease involved in post-transcriptional regulation of immune and inflammatory responses. Initially identified for its role in degrading mRNA transcripts of pro-inflammatory cytokines, RGN plays a critical role in maintaining immune homeostasis by controlling the stability of mRNAs encoding cytokines such as IL-6. IL-12. and TNF-α. Structurally, RGN contains a PilT N-terminal (PIN)-like ribonuclease domain, a CCCH-type zinc finger motif, and a ubiquitin-associated domain, enabling its dual functions in RNA cleavage and protein interactions.
RGN’s activity is tightly regulated through phosphorylation, ubiquitination, and proteasomal degradation. For instance, activation of TLR (Toll-like receptor) or IL-1R (interleukin-1 receptor) signaling pathways induces RGN phosphorylation, leading to its inactivation and subsequent accumulation of cytokine mRNAs to amplify immune responses. Conversely, RGN deficiency in mice results in severe autoimmune disorders, highlighting its role as a negative regulator of inflammation.
Recombinant RGN protein is engineered using expression systems like *E. coli* or mammalian cells to study its biochemical properties, RNA-binding specificity, and therapeutic potential. It serves as a tool for investigating mechanisms of mRNA decay, immune modulation, and inflammatory diseases. Recent studies explore RGN-based strategies to target aberrant cytokine production in conditions like rheumatoid arthritis, sepsis, and cancer.
In summary, RGN recombinant protein bridges fundamental research and clinical applications, offering insights into immune regulation and opportunities for developing anti-inflammatory therapies. Its unique ability to fine-tune cytokine expression positions it as a promising target for treating diseases driven by uncontrolled inflammation.
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