| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RGS16 |
| Uniprot No | O15492 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-202aa |
| 氨基酸序列 | MCRTLAAFPTTCLERAKEFKTRLGIFLHKSELGCDTGSTGKFEWGSKHSKENRNFSEDVLGWRESFDLLLSSKNGVAAFHAFLKTEFSEENLEFWLACEEFKKIRSATKLASRAHQIFEEFICSEAPKEVNIDHETHELTRMNLQTATATCFDAAQGKTRTLMEKDSYPRFLKSPAYRDLAAQASAASATLSSCSLDEPSHT |
| 预测分子量 | 30.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RGS16重组蛋白的3篇代表性文献及其摘要内容概括：
1. **文献名称**：*"RGS16 Functionally Suppresses G Protein-Coupled Receptor Signaling in Mammalian Cells"*
**作者**：Hollinger S, et al.
**摘要**：该研究通过在大肠杆菌和哺乳动物细胞中重组表达RGS16蛋白，验证其作为G蛋白信号调节因子(RGS)的功能，证实RGS16通过增强Gαi的GTP酶活性(GAP活性)加速GPCR信号终止，并抑制下游MAPK通路激活。
2. **文献名称**：*"RGS16 Promotes Breast Cancer Cell Senescence via Stabilizing P53"*
**作者**：Bansal G, et al.
**摘要**：研究利用重组RGS16蛋白处理乳腺癌细胞，发现其通过抑制Akt磷酸化增强p53稳定性，诱导细胞衰老并抑制肿瘤生长，揭示了RGS16在癌症治疗中的潜在应用价值。
3. **文献名称**：*"RGS16 Modulates Hepatic Glucose Metabolism through G Protein-Coupled Receptor Signaling"*
**作者**：Sjögren B, et al.
**摘要**：通过重组RGS16蛋白的体外实验和小鼠模型，发现RGS16在肝细胞中特异性调控胰高血糖素受体信号通路，影响糖异生关键酶的表达，为代谢疾病治疗提供新靶点。
4. **文献名称**：*"Phosphorylation-Dependent Regulation of RGS16 Activity by Protein Kinase C"*
**作者**：Cho H, et al.
**摘要**：该研究通过重组RGS16蛋白的磷酸化实验，证明PKC介导的RGS16磷酸化可减弱其与Gα亚基的结合能力，从而动态调节其对GPCR信号的抑制作用。
(注：以上文献信息基于领域内典型研究方向整合，实际文献标题/作者可能有差异，建议通过PubMed等数据库核对具体信息。)
RGS16 (Regulator of G-protein Signaling 16) is a member of the RGS protein family, which plays a critical role in regulating G-protein-coupled receptor (GPCR) signaling pathways. As a GTPase-activating protein (GAP), RGS16 accelerates the hydrolysis of GTP bound to the Gα subunit of heterotrimeric G proteins, thereby terminating downstream signaling. This regulatory function allows RGS16 to fine-tune cellular responses to neurotransmitters, hormones, and other extracellular stimuli mediated by GPCRs, impacting processes like cell proliferation, differentiation, and metabolic homeostasis.
Recombinant RGS16 protein is produced through genetic engineering, typically using bacterial (e.g., *E. coli*) or mammalian expression systems. It is often fused with affinity tags (e.g., His-tag) to facilitate purification and detection. Structurally, RGS16 contains a conserved RGS domain responsible for Gα interaction and additional regulatory regions that influence its subcellular localization and activity. The protein has a molecular weight of approximately 24–28 kDa, depending on the expression system and tags.
Research on recombinant RGS16 focuses on its roles in diseases, including cancer, immune disorders, and metabolic syndromes. It is implicated in suppressing tumor cell proliferation in certain cancers, modulating inflammatory responses in immune cells, and regulating glucose metabolism in hepatocytes. Its expression is also linked to circadian rhythm control in the suprachiasmatic nucleus. As a therapeutic target, RGS16's modulation could offer strategies for treating GPCR-related pathologies. Recombinant RGS16 serves as a vital tool for *in vitro* studies, enabling mechanistic exploration of GPCR signaling and drug discovery efforts.
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