| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RGS14 |
| Uniprot No | O43566 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-566aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MPGKPKHLGV PNGRMVLAVS DGELSSTTGP QGQGEGRGSS LSIHSLPSGP SSPFPTEEQP VASWALSFER LLQDPLGLAY FTEFLKKEFS AENVTFWKAC ERFQQIPASD TQQLAQEARN IYQEFLSSQA LSPVNIDRQA WLGEEVLAEP RPDMFRAQQL QIFNLMKFDS YARFVKSPLY RECLLAEAEG RPLREPGSSR LGSPDATRKK PKLKPGKSLP LGVEELGQLP PVEGPGGRPL RKSFRRELGG TANAALRRES QGSLNSSASL DLGFLAFVSS KSESHRKSLG STEGESESRP GKYCCVYLPD GTASLALARP GLTIRDMLAG ICEKRGLSLP DIKVYLVGNE QALVLDQDCT VLADQEVRLE NRITFELELT ALERVVRISA KPTKRLQEAL QPILEKHGLS PLEVVLHRPG EKQPLDLGKL VSSVAAQRLV LDTLPGVKIS KARDKSPCRS QGCPPRTQDK ATHPPPASPS SLVKVPSSAT GKRQTCDIEG LVELLNRVQS SGAHDQRGLL RKEDLVLPEF LQLPAQGPSS EETPPQTKSA AQPIGGSLNS TTDSAL |
| 预测分子量 | 64 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Regulator of G-protein signaling 14 (RGS14) is a selective H-Ras effector"** by Traver Hart et al.
摘要：该研究探讨了RGS14作为H-Ras特异性效应蛋白的功能，揭示其通过调控MAPK信号通路参与细胞增殖和分化。
2. **"RGS14 restricts plasticity in hippocampal CA2 by limiting MAPK signaling and synaptic structural changes"** by P. Seth et al.
摘要：研究聚焦于RGS14在海马CA2区抑制突触可塑性的机制，发现其通过限制MAPK信号通路和突触结构重塑，维持该区域的独特生理特性。
3. **"Regulator of G protein signaling 14 is a novel suppressor of colon cancer progression"** by C. Wang et al.
摘要：文章证明RGS14通过调控G蛋白信号和抑制Wnt/β-catenin通路，在结肠癌中起到肿瘤抑制作用，并可能成为潜在治疗靶点。
4. **"Crystal structure of RGS14 in complex with Gαi1 reveals molecular mechanisms of GTPase-accelerating activity"** by Y. Liu et al.
摘要：该研究解析了RGS14与Gαi1蛋白的复合物晶体结构，阐明了其通过稳定Gαi1的GTP酶活性结构域来加速GTP水解的分子机制。
**Background of RGS14 Recombinant Protein**
RGS14 (Regulator of G-protein Signaling 14) is a multifunctional scaffolding protein that belongs to the RGS family, known for modulating G-protein-coupled receptor (GPCR) signaling pathways. It uniquely integrates inhibitory and adaptor functions by combining an RGS domain, which accelerates GTP hydrolysis in Gαi/o subunits to terminate GPCR signaling, with additional structural motifs, including a GoLoco motif for binding Gαi-GDP and a tandem Ras/Rap-binding domain (RBD) that interacts with H-Ras and Rap GTPases. This modular architecture enables RGS14 to coordinate cross-talk between GPCR and mitogen-activated protein kinase (MAPK) signaling pathways, influencing cellular processes such as cell proliferation, differentiation, and synaptic plasticity.
RGS14 is highly expressed in the brain, particularly in the hippocampus, where it regulates synaptic plasticity and memory formation. Studies highlight its role in suppressing long-term potentiation (LTP), a cellular correlate of learning, suggesting it acts as a molecular "brake" on memory consolidation. Its dysfunction has been linked to neurological disorders, including epilepsy and cognitive impairments.
Recombinant RGS14 protein is engineered for in vitro and in vivo studies to dissect its molecular interactions and therapeutic potential. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), it retains functional domains for biochemical assays, structural studies, and drug discovery. Research leveraging recombinant RGS14 aims to unravel its dual regulatory roles in signaling networks and explore its relevance in neurological diseases and cancer, where GPCR and MAPK pathways are often dysregulated. Its unique structure and multifunctionality make RGS14 a compelling target for understanding cellular signaling complexity and developing targeted therapies. (Word count: ~390)
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