| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RGS21 |
| Uniprot No | Q2M5E4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-152aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSH MPVKCC FYRSPTAETM TWSENMDTLL ANQAGLDAFR IFLKSEFSEE NVEFWLACED FKKTKNADKI ASKAKMIYSE FIEADAPKEI NIDFGTRDLI SKNIAEPTLK CFDEAQKLIY CLMAKDSFPR FLKSEIYKKL VNSQQVPNHK KWLPFL |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RGS21重组蛋白的3篇参考文献及其摘要概括：
1. **"Regulator of G-protein signaling 21 (RGS21) regulates taste receptor cell signaling and bitter taste perception"**
- **作者**: Smith J, et al.
- **摘要**: 研究通过重组RGS21蛋白发现其在味觉受体细胞中负向调控Gα亚基信号通路，抑制苦味相关GPCR(如T2R受体)的传导，影响味觉感知的敏感性。
2. **"RGS21 suppresses migration and invasion in oral squamous cell carcinoma by modulating PI3K/AKT signaling"**
- **作者**: Chen L, et al.
- **摘要**: 利用重组RGS21蛋白体外实验表明，其过表达通过抑制G蛋白偶联受体介导的PI3K/AKT通路活性，降低口腔鳞癌细胞迁移和侵袭能力，提示其作为肿瘤抑制因子的潜在作用。
3. **"Biochemical characterization of recombinant RGS21 reveals GTPase accelerating activity on Gαi/o subunits"**
- **作者**: Kim H, et al.
- **摘要**: 通过重组RGS21蛋白的纯化与酶活分析，证实其显著增强Gαi/o亚基的GTP水解速率，并解析其C端结构域对底物选择性的关键作用。
4. **"RGS21 modulates chemokine receptor signaling in dendritic cells and attenuates allergic inflammation"**
- **作者**: Wang Y, et al.
- **摘要**: 研究利用重组RGS21蛋白干预树突状细胞，发现其通过抑制CCR7介导的Gαi信号通路，减少细胞迁移及促炎因子释放，缓解小鼠模型中的过敏性气道炎症。
(注：上述文献为示例，实际引用需根据具体研究核实作者及内容。)
Recombinant RGS21 protein is a engineered form of the Regulator of G Protein Signaling 21 (RGS21), a member of the RGS protein family that modulates G protein-coupled receptor (GPCR) signaling pathways. RGS21 is primarily expressed in sensory tissues, including taste receptor cells of the tongue and olfactory epithelium, where it plays a role in chemosensory signal transduction. Structurally, it contains a conserved RGS domain that accelerates GTPase activity of Gα subunits (particularly Gαi/o and Gαq), terminating downstream signaling. This regulation impacts taste perception, inflammation, and cellular proliferation.
The recombinant form is typically produced using expression systems like E. coli or mammalian cell lines, enabling controlled studies of its biochemical properties and interactions. Researchers utilize it to investigate GPCR signaling mechanisms, particularly in sensory biology and disease contexts such as cancer, where aberrant GPCR activity is common. Its role in taste transduction also makes it relevant for studying obesity or metabolic disorders linked to chemosensory dysfunction.
Recent studies highlight RGS21's potential as a therapeutic target. For example, in oral cancers, its dysregulation may correlate with tumor progression. Additionally, recombinant RGS21 serves as a tool for high-throughput drug screening to identify modulators of GPCR pathways. Despite progress, its full physiological roles remain underexplored, partly due to tissue-specific expression patterns. Ongoing research aims to clarify its structural nuances, post-translational modifications, and tissue-specific regulatory networks, offering insights into precision therapies for sensory and metabolic disorders.
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