| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RGS4 |
| Uniprot No | P49798 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-205aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMCKGLAGLPASCLRSAKDMKHRLGFLLQKS DSCEHNSSHNKKDKVVIC QRVSQEEVKKWAESLENLISHECGLAAFKA FLKSEYSEENIDFWISCEEYKKIKSPSKLSPKAKKIYNEFISVQA TKE VNLDSCTREETSRNMLEPTITCFDEAQKKIFNLMEKDSYRRFLKSRFYLD LVNPSSCGAEKQKGAKSSADCA SLVPQCA |
| 预测分子量 | 25 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RGS4重组蛋白的3篇示例参考文献(注：以下为模拟内容，实际文献需通过学术数据库查询)：
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1. **文献名称**: "Expression and purification of recombinant RGS4 in Escherichia coli: functional analysis of GAP activity"
**作者**: Smith A, et al.
**摘要**: 研究报道了RGS4在大肠杆菌系统中的高效表达与纯化方法，并验证其作为G蛋白信号调节因子(GAP)的活性，证明重组RGS4能加速Gαi亚基的GTP水解。
2. **文献名称**: "Crystal structure of RGS4 bound to Gαi1 reveals molecular determinants of GTPase regulation"
**作者**: Jones B, et al.
**摘要**: 通过X射线晶体学解析了重组RGS4与Gαi1的复合物结构，揭示了RGS4通过结合Gα亚基的“开关区”增强其GTP酶活性的分子机制。
3. **文献名称**: "RGS4 knockdown in neuronal cells alters G protein-coupled receptor signaling dynamics"
**作者**: Chen L, et al.
**摘要**: 利用重组RGS4蛋白及siRNA技术，研究其在神经元细胞中对GPCR信号通路(如多巴胺受体)的动态调控作用，表明RGS4缺失导致信号持续时间延长。
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如需真实文献，建议通过PubMed、Web of Science等平台搜索关键词“RGS4 recombinant protein”或结合具体研究领域筛选。
**Background of RGS4 Recombinant Protein**
Regulator of G-protein Signaling 4 (RGS4) is a member of the RGS protein family, which plays a critical role in modulating G-protein-coupled receptor (GPCR) signaling pathways. Discovered in the mid-1990s, RGS proteins act as GTPase-activating proteins (GAPs) for Gα subunits of heterotrimeric G-proteins, accelerating the hydrolysis of GTP to terminate signaling. RGS4 specifically targets Gαi and Gαq subunits, fine-tuning cellular responses to neurotransmitters, hormones, and other extracellular signals.
Structurally, RGS4 contains a conserved RGS domain responsible for Gα interaction, flanked by disordered N- and C-terminal regions that regulate its stability, localization, and protein interactions. Its expression is prominent in the brain, heart, and vascular tissues, implicating roles in neurological, cardiovascular, and psychiatric functions. Dysregulation of RGS4 has been linked to schizophrenia, hypertension, and heart failure, making it a potential therapeutic target.
Recombinant RGS4 protein is engineered through heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for research. It enables mechanistic studies of GPCR signaling, drug screening, and structural analyses (e.g., X-ray crystallography) to explore RGS4-Gα interactions. Researchers also utilize it to investigate post-translational modifications, such as phosphorylation, that modulate its activity.
Despite its utility, challenges persist in studying RGS4 due to its inherent instability and complex regulatory mechanisms. Ongoing work focuses on developing stabilized variants and small-molecule modulators to harness its therapeutic potential. Overall, RGS4 recombinant protein remains a vital tool for dissecting GPCR signaling dynamics and advancing drug discovery in related diseases.
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