| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RheB |
| Uniprot No | Q15382 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-181aa |
| 氨基酸序列 | MPQSKSRKIAILGYRSVGKSSLTIQFVEGQFVDSYDPTIENTFTKLITVN GQEYHLQLVDTAGQDEYSIFPQTYSIDINGYILVYSVTSIKSFEVIKVIH GKLLDMVGKVQIPIMLVGNKKDLHMERVISYEEGKALAESWNAAFLESSA KENQTAVDVFRRIILEAEKMDGAASQGKSSC |
| 预测分子量 | 23 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RheB重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*"Recombinant Rheb GTPase characterization and its interaction with mTORC1"*
**作者**：Inoki K. et al.
**摘要**：该研究通过在大肠杆菌中表达并纯化重组RheB蛋白，分析了其GTP/GDP结合特性及对哺乳动物雷帕霉素靶蛋白复合物1(mTORC1)的调控作用，证实RheB的活性状态直接激活mTORC1信号通路。
2. **文献名称**：*"Expression and functional analysis of RheB in Drosophila S2 cells"*
**作者**：Saucedo L.J. et al.
**摘要**：利用昆虫S2细胞系统表达重组RheB蛋白，研究发现其过表达显著促进细胞生长，并揭示了RheB通过调控mTOR通路影响细胞自噬和蛋白质合成的分子机制。
3. **文献名称**：*"Structural basis of Rheb-mTORC1 signaling revealed by X-ray crystallography"*
**作者**：Garami A. et al.
**摘要**：通过解析重组人源RheB蛋白的晶体结构，阐明了其与mTORC1关键亚基Raptor的结合模式，为靶向RheB-mTOR通路的药物设计提供了结构基础。
注：上述文献信息为示例性概括，实际文献需通过学术数据库检索确认。
**Background of RheB Recombinant Protein**
RheB (Ras homolog enriched in brain) is a small GTPase belonging to the Ras superfamily, primarily involved in regulating cellular growth, proliferation, and metabolism. It functions as a critical activator of the mechanistic target of rapamycin complex 1 (mTORC1), a central regulator of anabolic processes. RheB cycles between an active GTP-bound state and an inactive GDP-bound state, with its activity modulated by upstream signals, including growth factors, nutrients, and cellular stress. Dysregulation of RheB-mTORC1 signaling is implicated in various diseases, such as cancer, neurological disorders, and metabolic syndromes.
Recombinant RheB protein is engineered through genetic cloning and expression in systems like *E. coli*, yeast, or mammalian cells, enabling controlled production of functional, purified protein for research. Its recombinant form retains the ability to bind GTP/GDP and interact with downstream effectors, making it invaluable for studying mTORC1 pathway dynamics, protein-protein interactions, and structure-function relationships. Researchers utilize RheB recombinant protein to investigate its role in autophagy, cell survival, and responses to therapeutic agents. Additionally, it serves as a tool for screening inhibitors targeting RheB or mTORC1. with potential applications in drug development for cancers or mTOR-related disorders.
The use of recombinant RheB overcomes challenges associated with endogenous protein isolation, ensuring high purity and consistency. Mammalian-expressed RheB may include post-translational modifications critical for native functionality, enhancing its relevance in disease models. Studies leveraging recombinant RheB continue to advance understanding of cellular homeostasis and therapeutic targeting in pathological conditions.
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