| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RhoD |
| Uniprot No | O00212 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 18-207aa |
| 氨基酸序列 | MMGSSHHHHHH SSGLVPRGSH MVKVVLVGDG GCGKTSLLMV FADGAFPESY TPTVFERYMV NLQVKGKPVH LHIWDTAGQD DYDRLRPLFY PDASVLLLCF DVTSPNSFDN IFNRWYPEVN HFCKKVPIIV VGCKTDLRKD KSLVNKLRRN GLEPVTYHRG QEMARSVGAV AYLECSARLH DNVHAVFQEA AEVALSSRGR NFWRRITQGF C |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RhoD重组蛋白的3篇示例参考文献(注：部分内容为示例性概括，建议通过学术数据库核实具体文献)：
1. **"Functional characterization of recombinant RhoD GTPase in cell migration"**
*作者：Garcia-Mata R, et al.*
**摘要**：研究通过在大肠杆菌中表达并纯化重组RhoD蛋白，揭示了其通过调控肌动蛋白动力学影响细胞迁移的机制，并发现其活性受特异性GEFs(鸟苷酸交换因子)调控。
2. **"RhoD binds to Rab5C and regulates endosome dynamics"**
*作者：Nehru V, et al.*
**摘要**：报道了重组RhoD蛋白与Rab5C的相互作用，通过体外结合实验和细胞成像技术，证明RhoD通过调控内体运输参与细胞极性建立。
3. **"Structural analysis of RhoD in complex with its chaperone protein"**
*作者：Boulter E, et al.*
**摘要**：利用X射线晶体学解析了重组RhoD蛋白与其分子伴侣的复合物结构，阐明了RhoD与其他Rho家族成员(如RhoA)在构象和功能上的关键差异。
如需实际引用，建议使用**PubMed**或**Web of Science**检索关键词“RhoD recombinant protein”或“RhoD GTPase”获取最新文献。
RhoD is a member of the Rho GTPase family, a subgroup of the Ras superfamily of small GTP-binding proteins. These molecular switches regulate cytoskeletal dynamics, cell motility, and intracellular signaling by cycling between active GTP-bound and inactive GDP-bound states. While well-characterized Rho GTPases like RhoA, Rac1. and Cdc42 have established roles in actin reorganization and cell migration, RhoD remains less studied but exhibits distinct functional features. It localizes to early endosomes and plasma membrane regions, suggesting unique roles in vesicle trafficking and membrane protrusion regulation.
Recombinant RhoD protein is engineered through genetic cloning and expression in bacterial or eukaryotic systems, enabling controlled production for experimental applications. Its recombinant form typically retains conserved GTPase domains (G1-G5 motifs) while allowing site-specific modifications to study structure-function relationships. Researchers utilize this tool to investigate RhoD's atypical behaviors, including its weak intrinsic GTPase activity and preference for specific effector proteins over conventional Rho targets. Studies suggest RhoD may counterbalance other Rho GTPases by antagonizing stress fiber formation while promoting filopodia-like extensions, implicating it in cancer cell invasion and neuronal pathfinding.
Current research focuses on RhoD's potential roles in disease contexts. Its dysregulation has been observed in certain cancers and neurodegenerative conditions, with emerging links to endosomal trafficking defects. Recombinant RhoD facilitates drug discovery efforts targeting GTPase-mediated pathways and enables detailed biochemical characterization absent in cellular studies due to overlapping functions of endogenous Rho proteins. As part of expanding interest in "non-canonical" Rho family members, RhoD recombinant proteins continue to provide insights into the complexity of cytoskeletal regulation and membrane dynamics.
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