首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠神经胶质纤维酸性蛋白(GFAP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GFAP含量。GFAP 即胶质纤维酸性蛋白，是星形胶质细胞的一种中间丝蛋白。其表达变化与神经系统损伤、疾病密切相关。研究中常将其作为星形胶质细胞活化的标志物，通过检测其水平来评估神经损伤程度、疾病进展等，为相关疾病诊疗提供依据。试剂盒检测范围为3.12 pg/mL-200 pg/mL，可稳定检测小鼠模型中创伤性脑损伤、阿尔茨海默病等疾病相关胶质细胞反应动态，亦适用于神经毒性化合物筛选或神经保护剂疗效评估等基础研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：GfapGlial fibrillary acidic protein ELISA Kit; GFAP ELISA Kit
缩写：GFAP
Uniprot No.：P03995
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse GFAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 95-101 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 97-108 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 91-97 | |||||
回收率：
| The recovery of mouse GFAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 91-104 | ||||
| EDTA plasma (n=4) | 93 | 90-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.5632.5952.5792.4001001.6361.7641.7001.521501.0271.0951.0610.882250.7040.6670.6860.50712.50.3870.3590.3730.1946.250.2860.2840.2850.1063.120.2330.2220.2280.04900.1730.1840.179 | ||||||
货期：3-5 working days
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