首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人γ谷氨酰水解酶(GGH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的GGH含量。试剂盒检测范围为0.45 ng/mL-30 ng/mL，本试剂盒适用于基础科研领域，可支持癌症机制研究、药物代谢动力学分析、神经退行性疾病模型构建等实验，尤其适用于对比不同组织或细胞模型中 GGH 表达水平的变化，为探索其在细胞周期调控和氧化应激反应中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Conjugase ELISA Kit; folylpolygammaglutamyl hydrolase ELISA Kit; gamma glutamyl hydrolase ELISA Kit; Gamma-Glu-X carboxypeptidase ELISA Kit; Gamma-glutamyl hydrolase (conjugase; folylpolygammaglutamyl hydrolase) ELISA Kit; Gamma-glutamyl hydrolase ELISA Kit; GGH ELISA Kit; GGH_HUMAN ELISA Kit; gH ELISA Kit
缩写：GGH
Uniprot No.：Q92820
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.45 ng/mL-30 ng/mL
灵敏度：0.11 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GGH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 84-94 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-100 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 86-95 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 89-100 | |||||
回收率：
| The recovery of human GGH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 88-97 | ||||
| EDTA plasma (n=4) | 103 | 96-106 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected302.7282.6672.6982.582151.5521.4781.5151.3997.50.7610.7540.7580.6423.750.5050.4990.5020.3861.80.3150.3230.3190.2030.90.2380.2420.2400.1240.450.1970.1920.1950.07900.1180.1140.116 | ||||||
货期：3-5 working days
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