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Dog growth hormone,GH ELISA Kit

  • 中文名: 犬生长激素(GH)ELISA Kit
货号: IPD-E14962c
Price: ¥2500
数量:
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产品详情

  • 产品描述:
    犬生长激素(GH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的GH1含量。GH1 即生长激素 1 基因,它编码生长激素,在人体生长发育、代谢等生理过程中扮演重要角色。研究机制方面,主要围绕其调控生长激素合成与分泌、与生长信号通路的相互作用等,这些研究有助于深入理解生长发育异常机制及相关疾病治疗。试剂盒检测范围为0.625 ng/mL-40 ng/mL,该产品适用于动物生长机制研究、代谢调控分析、药物或营养干预实验等科研领域,为探究犬类生理病理过程中的GH动态变化提供稳定工具本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
  • 别名:
    GH1 ELISA Kit; GH ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
  • 缩写:
    GH1
  • Uniprot No.:
    P33711
  • 种属:
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型:
    serum, plasma, tissue homogenates
  • 检测范围:
    0.625 ng/mL-40 ng/mL
  • 灵敏度:
    0.156 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Signal Transduction
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:

    Intra-assay Precision (Precision within an assay): CV%<8%




    Three samples of known concentration were tested twenty times on one plate to assess.


    Inter-assay Precision (Precision between assays): CV%<10%




    Three samples of known concentration were tested in twenty assays to assess.



  • 线性度:

    To assess the linearity of the assay, samples were spiked with high concentrations of dog GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.


    Sample

    Serum(n=4)


    1:1

    Average %

    92


    Range %

    88-97


    1:2

    Average %

    98


    Range %

    91-105


    1:4

    Average %

    89


    Range %

    84-95


    1:8

    Average %

    99


    Range %

    92-106


  • 回收率:

    The recovery of dog GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

    Sample Type

    Average % Recovery

    Range


    Serum (n=5)

    92

    85-99


    EDTA plasma (n=4)

    99

    92-106


  • 标准曲线:

    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.







    ng/ml

    OD1

    OD2

    Average

    Corrected


    40

    2.853

    2.734

    2.794

    2.632


    20

    2.087

    2.067

    2.077

    1.916


    10

    1.504

    1.498

    1.501

    1.339


    5

    0.897

    0.929

    0.913

    0.751


    2.5

    0.550

    0.521

    0.536

    0.374


    1.25

    0.383

    0.363

    0.373

    0.211


    0.625

    0.271

    0.284

    0.277

    0.116


    0

    0.158

    0.165

    0.162




  • 货期:
    3-5 working days

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