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产品描述：山羊生长激素(GH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GH1含量。GH1 编码生长激素，其对人体生长发育至关重要。研究机制方面，GH1 表达产物生长激素主要通过与受体结合，激活 JAK-STAT 等信号通路，来调节细胞增殖、分化和代谢，影响骨骼、肌肉等组织生长，目前围绕它的研究有望为生长相关疾病治疗提供思路。试剂盒检测范围为6.25 ng/mL-100 ng/mL，本产品适用于科研领域，如动物生理学研究、内分泌机制探索、畜牧业生长调控优化或药物开发中GH相关生物标志物分析，其检测可为研究山羊生长发育、代谢疾病模型建立及营养干预效果评估提供重要依据。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：GH1 ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
缩写：GH1
Uniprot No.：P67931
种属：Capra hircus (Goat)
样本类型：serum, plasma, tissue homogenates
检测范围：6.25 ng/mL-100 ng/mL
灵敏度：6.25 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of goat GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 84-90 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 88-97 | |||||
回收率：
| The recovery of goat GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-99 | ||||
| EDTA plasma (n=4) | 94 | 89-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 1000.2020.2000.201 500.3960.3650.381 250.6310.6220.627 12.51.1421.1541.148 6.252.3592.3262.343 | ||||||
货期：3-5 working days
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