首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠促生长激素释放激素(GHRH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GHRH含量。GHRH（生长激素释放激素）是一种由下丘脑分泌的肽类激素，主要作用是调节生长激素的释放和神经功能。它通过激活G蛋白偶联受体，激活cAMP信号通路，促进细胞增殖、生长激素合成与分泌，并参与免疫系统调节、细胞分化、细胞增殖等生物学过程。试剂盒检测范围为3.12 pg/mL-200 pg/mL，本产品广泛应用于神经内分泌研究、生长激素相关疾病模型构建、药物干预效果评价等科研领域，为探索GHRH在生长发育、代谢疾病或应激反应中的调控机制提供可靠工具。试剂盒包含完整实验组分，支持常规实验室操作流程，适用于大鼠模型的基础研究与转化医学探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ghrh ELISA Kit; Somatoliberin ELISA Kit; Growth hormone-releasing factor ELISA Kit; GRF ELISA Kit; Growth hormone-releasing hormone ELISA Kit; GHRH ELISA Kit
缩写：GHRH
Uniprot No.：P09916
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat GHRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 97 | |||||
| Range % | 92-103 | ||||||
| 1:2 | Average % | 92 | |||||
| Range % | 85-99 | ||||||
| 1:4 | Average % | 90 | |||||
| Range % | 88-94 | ||||||
| 1:8 | Average % | 101 | |||||
| Range % | 96-105 | ||||||
回收率：
| The recovery of rat GHRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 93 | 87-95 | |||||
| EDTA plasma (n=4) | 106 | 103-111 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected2002.0882.2522.1702.0801001.5851.6761.6311.541501.1631.1041.1341.044250.8310.7650.7980.70812.50.5500.5240.5370.4476.250.3440.3450.3450.2553.120.1820.1830.1830.09300.0900.0890.090 | |||||||
货期：3-5 working days
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