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产品描述：大鼠饥饿激素(GHRL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的GHRL含量。GHRL是胃饥饿素的编码基因。其背景上，胃饥饿素是一种主要由胃分泌的肽类激素。研究机制方面，它与调节食欲、能量代谢等密切相关，能作用于下丘脑等部位，影响神经内分泌系统，进而调控机体的摄食行为和能量平衡。试剂盒检测范围为7.8 pg/mL-500 pg/mL，适用于研究能量代谢调控、摄食行为机制、肥胖相关疾病模型评估，以及药物干预下激素分泌动态分析等科研场景。通过标准化的实验流程，研究人员可高效获取重复性良好的定量数据，为探索GHRL在神经内分泌调控及代谢性疾病中的作用提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ghrl ELISA Kit; Appetite-regulating hormone ELISA Kit; Growth hormone secretagogue ELISA Kit; Growth hormone-releasing peptide ELISA Kit; Motilin-related peptide) [Cleaved into: Ghrelin; Obestatin-23; Obestatin-13] ELISA Kit
缩写：GHRL
Uniprot No.：Q9QYH7
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat GHRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:10 | Average % | 98 | ||||
| Range % | 95-103 | |||||
| 1:20 | Average % | 101 | ||||
| Range % | 95-106 | |||||
| 1:40 | Average % | 94 | ||||
| Range % | 85-99 | |||||
回收率：
| The recovery of rat GHRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 90-102 | ||||
| EDTA plasma (n=4) | 93 | 89-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.6372.6912.6642.4692502.2172.2582.2382.0431251.5231.5891.5561.36162.50.9650.9740.9700.77531.20.5460.5910.5690.37415.60.3570.3690.3630.1687.80.2980.2740.2860.09100.1940.1960.195 | ||||||
货期：3-5 working days
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