首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠葡萄糖依赖性胰岛素释放多肽(GIP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GIP含量。GIP（葡萄糖依赖性促胰岛素释放多肽）是一种肠促胰岛素激素，参与糖代谢和脂代谢，通过多种途径增加胰岛素敏感性，改善胰岛素抵抗。其研究机制涉及调节脂肪细胞代谢、促进β细胞增殖等，对治疗2型糖尿病和肥胖具有重要意义。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于科研领域探索GIP在糖代谢调控、胰岛素分泌及能量平衡中的作用机制。该产品适用于小鼠模型中GIP与糖尿病、肥胖症或胃肠道功能相关的基础研究，实验结果需结合其他代谢指标进行综合分析，严禁用于临床诊断。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：GipGastric inhibitory polypeptide ELISA Kit; GIP ELISA Kit; Glucose-dependent insulinotropic polypeptide ELISA Kit
缩写：GIP
Uniprot No.：P48756
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse GIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 88 | |||||
| Range % | 84-92 | ||||||
| 1:2 | Average % | 97 | |||||
| Range % | 94-101 | ||||||
| 1:4 | Average % | 102 | |||||
| Range % | 97-108 | ||||||
| 1:8 | Average % | 94 | |||||
| Range % | 89-98 | ||||||
回收率：
| The recovery of mouse GIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 102 | 98-107 | |||||
| EDTA plasma (n=4) | 93 | 82-97 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected 102.1342.2572.1962.080 51.6751.7391.7071.591 2.51.1441.1851.1651.049 1.250.6720.6960.6840.568 0.6250.4040.4230.4140.298 0.3120.2880.2930.2910.175 0.1560.2020.2090.2060.090 00.1150.1170.116 | |||||||
货期：3-5 working days
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