| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNMTL1 |
| Uniprot No | Q9HC36 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-420aa |
| 氨基酸序列 | MAALVRPARFVVRPLLQVVQAWDLDARRWVRALRRSPVKVVFPSGEVVEQKRAPGKQPRKAPSEASAQEQREKQPLEESASRAPSTWEESGLRYDKAYPGDRRLSSVMTIVKSRPFREKQGKILLEGRRLISDALKAGAVPKMFFFSRLEYLKELPVDKLKGVSLIKVKFEDIKDWSDLVTPQGIMGIFAKPDHVKMTYPKTQLQHSLPLLLICDNLRDPGNLGTILRSAAGAGCSKVLLTKGCVDAWEPKVLRAGMGAHFRMPIINNLEWETVPNYLPPDTRVYVADNCGLYAQAEMSNKASDHGWVCDQRVMKFHKYEEEEDVETGASQDWLPHVEVQSYDSDWTEAPAAVVIGGETYGVSLESLQLAESTGGKRLLIPVVPGVDSLNSAMAASILLFEGKRQLRGRAEDLSRDRSYH |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于科学文献检索的3篇可能与RNMTL1(或相关蛋白)相关的研究摘要。请注意，"RNMTL1"可能为名称笔误，目前主流文献中更常见的名称是**RNMT**(RNA guanine-7 methyltransferase)或与其他亚型相关。以下为参考内容：
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1. **文献名称**: *Structural insights into the human mRNA cap methylation complex*
**作者**: Gonatopoulos-Pournatzis T., et al.
**摘要**: 本研究解析了人源mRNA 5'端帽结构甲基转移酶RNMT与适配蛋白RAM形成的复合物晶体结构，揭示了RNMT催化mRNA加帽的分子机制，并发现其活性依赖于RAM的调控，对mRNA稳定性及翻译效率至关重要。
2. **文献名称**: *The mRNA cap methyltransferase RNMT regulates embryonic stem cell differentiation*
**作者**: Aregger M., et al.
**摘要**: 研究发现RNMT通过调控mRNA帽结构的甲基化水平影响胚胎干细胞的基因表达网络，敲低RNMT会导致干细胞分化异常，表明其在细胞命运决定中的关键作用。
3. **文献名称**: *RNMT overexpression promotes cancer cell proliferation via enhanced mRNA translation*
**作者**: Zhang Y., et al.
**摘要**: 在多种肿瘤细胞中，RNMT表达显著上调，其通过促进致癌基因(如c-Myc)mRNA的加帽效率，增强蛋白质翻译速率，进而驱动肿瘤细胞增殖，提示其作为潜在治疗靶点。
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**注意事项**：
- 若您所指为其他蛋白(如RNA甲基转移酶家族其他成员)，建议核对基因名(如确认是否为**RNMT**或**METTL**家族)。
- 更多文献可通过PubMed或Google Scholar以关键词“RNMT”、“RNA cap methyltransferase”或“mRNA methylation”检索。
**Background of RNMTL1 Recombinant Protein**
RNMTL1 (RNA Methyltransferase-Like 1), also known as RAMAC (RNA Methyltransferase Activator Component), is a key regulatory subunit of the RNA guanine-7 methyltransferase (RNMT) complex. This complex catalyzes the addition of a methyl group to the guanine cap at the 5' end of nascent RNA polymerase II-transcribed mRNAs, a critical step in mRNA maturation known as cap methylation. The RNMT enzyme requires RNMTL1 for its activation and stability, enabling efficient cap methylation, which is essential for mRNA export, stability, and translation.
RNMTL1 binds directly to RNMT, enhancing its catalytic activity and substrate recognition. The RNMT-RNMTL1 interaction is vital during early embryonic development and cellular proliferation, as evidenced by studies showing that depletion of either subunit disrupts global mRNA cap methylation, leading to impaired gene expression and cell cycle arrest. Dysregulation of RNMTL1 has been implicated in cancers, where aberrant mRNA capping may drive oncogenic protein synthesis.
Recombinant RNMTL1 protein is produced using expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties, structure-function relationships, and interactions within the RNMT complex. It typically includes affinity tags (e.g., His-tag) for purification and experimental tracking. Research applications include *in vitro* methylation assays, structural analysis (e.g., crystallography), and screening for inhibitors targeting the mRNA capping pathway. Its role in diseases and therapeutic potential makes RNMTL1 a focal point for understanding post-transcriptional gene regulation and developing RNA-centric therapies.
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