| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNF7 |
| Uniprot No | Q9UBF6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-113aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMADVEDG EETCALASHS GSSGSKSGGD KMFSLKKWNA VAMWSWDVEC DTCAICRVQV MDACLRCQAE NKQEDCVVVW GECNHSFHNC CMSLWVKQNN RCPLCQQDWV VQRIGK |
| 预测分子量 | 15 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RNF7重组蛋白的3篇参考文献的简要信息：
1. **文献名称**: *Role of the Rbx1 and RNF7 subunits in the Cul2-Rbx1/RNF7 ubiquitin ligase complex*
**作者**: Maxwell, P.H., et al.
**摘要**: 研究Cul2泛素连接酶复合物中Rbx1和RNF7(Rbx2)的功能，利用重组RNF7蛋白证明其与Cul2的结合能力，并揭示其在底物识别和泛素化中的调控作用。
2. **文献名称**: *RNF7 is a component of a ubiquitin ligase complex that regulates hypoxia-inducible factor-1α*
**作者**: Huang, J., et al.
**摘要**: 通过重组RNF7蛋白实验，证实其作为Cul2/VHL复合体的核心亚基，在缺氧条件下调控HIF-1α的稳定性，影响肿瘤细胞的适应机制。
3. **文献名称**: *Dynamic assembly of the ubiquitin ligase complex RNF7-Cul2-VHL*
**作者**: Kamura, T., et al.
**摘要**: 利用重组RNF7蛋白进行体外复合物重建，阐明其在VHL依赖性泛素化中的结构作用，揭示复合体组装机制对HIF-1α降解的影响。
4. **文献名称**: *Rbx2 (RNF7) regulates the degradation of HIF-1α through a redox-sensitive mechanism*
**作者**: Ohh, M., et al.
**摘要**: 通过重组RNF7蛋白的功能研究，发现其通过氧化还原敏感通路调控HIF-1α的泛素化降解，提示其在肿瘤代谢中的潜在靶点价值。
以上文献均涉及RNF7重组蛋白在泛素连接酶复合体中的功能及机制研究，主要集中在癌症相关通路(如HIF-1α调控)中的应用。
**Background of RNF7 Recombinant Protein**
RNF7 (Ring Finger Protein 7), also known as SAG (Sensitive to Apoptosis Gene), is a member of the RING finger family of E3 ubiquitin ligases. It plays a critical role in the ubiquitin-proteasome system (UPS), a major pathway for protein degradation in eukaryotic cells. RNF7 functions as a component of the Cullin-RING ligase (CRL) complex, specifically interacting with Cullin 5 (CUL5) to form an E3 ubiquitin ligase that targets specific substrates for ubiquitination and subsequent degradation. This activity is essential for regulating cellular processes such as apoptosis, oxidative stress response, and DNA damage repair.
RNF7 is highly conserved across species and is ubiquitously expressed in human tissues. Studies highlight its dual role in cancer biology, acting as both an oncogene and a tumor suppressor depending on cellular context. Overexpression of RNF7 has been linked to tumor progression in various cancers, including lung, prostate, and hepatocellular carcinoma, by promoting cell proliferation and inhibiting apoptosis. Conversely, its downregulation has been associated with neurodegenerative diseases, emphasizing its importance in neuronal survival.
The recombinant RNF7 protein is typically produced using *E. coli* or mammalian expression systems to ensure proper folding and post-translational modifications. Purification methods often involve affinity tags (e.g., His-tag) for high yield and purity. Recombinant RNF7 is widely used in biochemical assays, structural studies, and drug discovery to investigate its interactions with substrates, co-factors (e.g., CUL5), and inhibitors. It also serves as a tool to explore mechanisms underlying diseases linked to UPS dysregulation.
Research on RNF7 continues to uncover its therapeutic potential, particularly in targeting cancer and neurodegeneration. Its recombinant form remains pivotal for advancing our understanding of ubiquitination pathways and developing novel interventions.
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