| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNF34 |
| Uniprot No | Q969K3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-372aa |
| 氨基酸序列 | MKAGATSMWASCCGLLNEVMGTGAVRGQQSAFAGATGPFRFTPNPEFSTY PPAATEGPNIVCKACGLSFSVFRKKHVCCDCKKDFCSVCSVLQENLRRCS TCHLLQETAFQRPQLMRLKVKDLRQYLILRNIPIDTCREKEDLVDLVLCH HGLGSEDDMDTSSLNSSRSQTSSFFTRSFFSNYTAPSATMSSFQGELMDG DQTSRSGVPAQVQSEITSANTEDDDDDDDEDDDDEEENAEDRNPGLSKER VRASLSDLSSLDDVEGMSVRQLKEILARNFVNYSGCCEKWELVEKVNRLY KENEENQKSYGERLQLQDEEDDSLCRICMDAVIDCVLLECGHMVTCTKCG KRMSECPICRQYVVRAVHVFKS |
| 预测分子量 | 68 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于RNF34重组蛋白的模拟参考文献示例(注：文献为虚构示例，实际研究需核实真实文献)：
1. **文献名称**: "Structural and Functional Analysis of RNF34 Ubiquitin Ligase"
**作者**: Li X, et al.
**摘要**: 解析了RNF34重组蛋白的晶体结构，发现其通过RING结构域介导E2泛素结合酶招募，揭示了其在泛素化底物识别中的特异性机制，并证实其通过蛋白酶体途径调控靶蛋白降解。
2. **文献名称**: "RNF34 Regulates p53 Stability via Ubiquitination in DNA Damage Response"
**作者**: Wang Y, et al.
**摘要**: 研究通过重组RNF34蛋白体外实验，证明其直接泛素化修饰p53.调控p53在DNA损伤后的稳定性，揭示了RNF34在细胞应激反应中的双重调节作用。
3. **文献名称**: "RNF34 Recombinant Protein Expression and Interaction Screening"
**作者**: Gupta S, et al.
**摘要**: 开发了高效表达纯化RNF34重组蛋白的原核系统，结合质谱技术筛选出与RNF34相互作用的凋亡相关蛋白Bcl-2家族成员，为研究其生理功能提供工具。
4. **文献名称**: "RNF34 Knockdown and Overexpression in Cancer Cell Models"
**作者**: Martinez R, et al.
**摘要**: 利用重组RNF34蛋白研究其在乳腺癌细胞中的功能，发现高表达RNF34通过泛素化降解促凋亡因子抑制细胞死亡，提示其作为潜在治疗靶点。
(提示：以上为模拟文献，实际文献需通过PubMed/Google Scholar检索关键词如"RNF34 recombinant protein"、"RNF34 ubiquitin ligase"获取。)
**Background of RNF34 Recombinant Protein**
RNF34 (Ring Finger Protein 34) is a member of the RING (Really Interesting New Gene) finger protein family, characterized by a conserved zinc-binding RING domain that confers E3 ubiquitin ligase activity. This enzyme plays a critical role in the ubiquitin-proteasome system (UPS), mediating the transfer of ubiquitin molecules to target proteins, marking them for degradation or functional modulation. RNF34 is involved in diverse cellular processes, including apoptosis, DNA damage response, immune regulation, and protein quality control.
Recombinant RNF34 protein is engineered through molecular cloning, typically expressed in *E. coli* or mammalian cell systems, to ensure proper folding and post-translational modifications. The purified protein retains its functional RING domain, enabling researchers to study its enzymatic activity, substrate interactions, and regulatory mechanisms *in vitro*. Structurally, RNF34 contains an N-terminal transmembrane domain, a central disordered region, and a C-terminal RING domain, which facilitates interactions with E2 ubiquitin-conjugating enzymes.
Studies highlight RNF34's role in apoptosis by promoting the degradation of anti-apoptotic proteins like BCL2L10 and RIPK1. It also regulates immune responses by modulating NF-κB signaling and participates in viral defense pathways. Dysregulation of RNF34 is linked to cancers, neurodegenerative diseases, and immune disorders, making it a potential therapeutic target.
The availability of recombinant RNF34 protein accelerates mechanistic studies, high-throughput screening for drug discovery, and structural analysis to elucidate its role in disease pathogenesis. Its utility extends to investigating ubiquitination cascades and developing targeted therapies aimed at modulating UPS-related pathways.
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