首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人α半乳糖苷酶(αGAL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GLA含量。GLA即α-半乳糖苷酶A，相关背景上，其缺陷会引发法布里病。在研究机制方面，主要探索补充该酶的治疗方式，通过提供外源性GLA改善机体代谢，减轻因底物蓄积造成的多器官损害，为法布里病治疗带来希望。试剂盒检测范围为7.8 pg/mL-500 pg/mL，该产品适用于法布雷病机制研究、溶酶体贮积症模型构建、药物干预效果评价等科研领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AGAL_HUMAN ELISA Kit; Agalsidase alfa ELISA Kit; Alpha D galactosidase A ELISA Kit; Alpha D galactoside galactohydrolase 1 ELISA Kit; Alpha D galactoside galactohydrolase ELISA Kit; Alpha gal A ELISA Kit; Alpha galactosidase A ELISA Kit; Alpha-D-galactosidase A ELISA Kit; Alpha-D-galactoside galactohydrolase ELISA Kit; Alpha-galactosidase A ELISA Kit; GALA ELISA Kit; Galactosidase; alpha ELISA Kit; GLA ELISA Kit; GLA protein ELISA Kit; Melibiase ELISA Kit
缩写：GLA
Uniprot No.：P06280
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human αGAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 86 | ||||
| Range % | 81-92 | |||||
| 1:200 | Average % | 92 | ||||
| Range % | 87-96 | |||||
| 1:400 | Average % | 84 | ||||
| Range % | 81-86 | |||||
| 1:800 | Average % | 102 | ||||
| Range % | 100-106 | |||||
回收率：
| The recovery of human αGAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 88 | 84-93 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.4202.4512.4362.2722502.0652.1072.0861.9221251.6631.7051.6841.52062.51.1651.1831.1741.01031.20.7350.7880.7620.59815.60.4820.4990.4910.3277.80.3280.3410.3350.17100.1620.1660.164 | ||||||
货期：3-5 working days
×
