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产品描述：牛促性腺激素释放激素(GnRH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GnRH含量。GnRH（促性腺激素释放激素）是一种由下丘脑分泌的激素，主要调节生殖系统的功能。其研究背景涉及生殖调控、内分泌系统以及肿瘤治疗等领域。GnRH通过作用于垂体前叶，调节促性腺激素的分泌，进而影响性腺发育和生殖过程。研究机制主要围绕GnRH的受体、信号通路及其在不同疾病中的作用展开。试剂盒检测范围为100 pg/mL-4000 pg/mL，适用于研究牛生殖周期调控、繁殖力评估、激素分泌动态监测等科研场景，特别适用于畜牧业中生殖内分泌机制探索及生殖健康相关研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：GnRH
Uniprot No.：Q0VBW7
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates
检测范围：100 pg/mL-4000 pg/mL
灵敏度：100 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine GnRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 83-95 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-101 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-101 | |||||
| 1:8 | Average % | 90 | ||||
| Range % | 85-98 | |||||
回收率：
| The recovery of bovine GnRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-98 | ||||
| EDTA plasma (n=4) | 97 | 94-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average02.5132.5272.5201001.0421.0321.0373000.5220.5100.5169200.3800.3640.37220000.2610.2460.25440000.1320.1340.133 | ||||||
货期：3-5 working days
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