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产品描述：鱼类促性腺激素释放激素(GnRH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆、细胞裂解物样本中的GnRH含量。GnRH即促性腺激素释放激素，是下丘脑分泌的十肽激素。它作用于腺垂体，调节促性腺激素合成与释放，进而调控性腺功能。研究围绕其分泌调节机制、与受体相互作用、信号转导通路等展开，以探索其在生殖内分泌疾病治疗中的应用。试剂盒检测范围为0.5 ng/mL-20 ng/mL，科研人员可借助本试剂盒开展鱼类生殖生理机制研究、水产养殖中人工繁殖技术优化、环境激素干扰评估等实验，例如监测不同发育阶段或环境胁迫下鱼类GnRH的动态变化，分析内分泌调控网络对繁殖行为的影响，为水生生物资源保护及可持续养殖提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：GnRH
Uniprot No.：Q5Y835
种属：Fish
样本类型：serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：0.5 ng/mL-20 ng/mL
灵敏度：0.5 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of fish GnRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 93-104 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 85-103 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 83-102 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 87-101 | |||||
回收率：
| The recovery of fish GnRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-94 | ||||
| EDTA plasma (n=4) | 94 | 89-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 200.1340.1400.137 100.2320.2420.237 4.60.3980.4090.404 1.50.7140.6870.701 0.51.2931.2661.280 02.7112.7402.726 | ||||||
货期：3-5 working days
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