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产品描述：山羊促性腺激素释放激素(GnRH)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GnRH含量。GnRH（促性腺激素释放激素）是下丘脑分泌的十肽激素，在生殖调节中起关键作用。其作用机制为脉冲式分泌，刺激垂体前叶合成和释放促性腺激素，进而调控性腺功能。对GnRH的研究有助于了解生殖生理及攻克相关疾病。试剂盒检测范围为2.5 pg/mL-500 pg/mL，该产品可广泛应用于山羊等反刍动物的生殖周期研究、繁殖调控机制探索、激素水平动态监测以及环境内分泌干扰物评估等科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：GnRH
Uniprot No.：D3YJ02
种属：Capra hircus (Goat)
样本类型：serum, plasma, tissue homogenates
检测范围：2.5 pg/mL-500 pg/mL
灵敏度：1.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of goat GnRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 88-102 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-99 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 89-103 | |||||
回收率：
| The recovery of goat GnRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 88-102 | ||||
| EDTA plasma (n=4) | 97 | 91-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average02.5572.4582.5082.51.4951.4811.488101.0531.0451.049400.6500.6360.6431250.4080.3920.4005000.2220.2140.218 | ||||||
货期：3-5 working days
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