首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人糖原磷酸化酶同工酶BB(GP-BB)酶联免疫试剂盒 双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GP-BB含量。试剂盒检测范围为0.625 ng/mL-40 ng/mL，本产品适用于科研领域中心肌损伤相关机制研究。该产品可用于心肌缺血动物模型评估、心脏疾病机制研究以及药物干预效果分析等科研场景，为体外实验提供定量数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：GP-BB
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GP-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 85-98 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 90-102 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-101 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 84-102 | |||||
回收率：
| The recovery of human GP-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-100 | ||||
| EDTA plasma (n=4) | 96 | 92-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected403.0473.1083.0782.901202.4752.5572.5162.339101.8391.7851.8121.63551.1281.1241.1260.9492.50.7240.7190.7220.5451.250.4960.4680.4820.3050.6250.3300.3320.3310.15400.1770.1760.177 | ||||||
货期：3-5 working days
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