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产品描述:人血小板糖蛋白VI(GP6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的GP6含量。GP6是一种血小板膜糖蛋白,编码免疫球蛋白超家族的血小板膜糖蛋白。其蛋白质是胶原蛋白的受体,在胶原蛋白诱导的血小板聚集和血栓形成中起着关键作用。研究GP6有助于理解血小板功能及其在血栓形成中的作用机制。试剂盒检测范围为46.88 pg/mL-3000 pg/mL,适用于体外实验环境下探究血小板相关疾病的分子机制、药物筛选或基础医学研究中GP6表达水平的评估本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
别名:Glycoprotein 6 ELISA Kit; Glycoprotein VI ELISA Kit; GP6 ELISA Kit; GPIV ELISA Kit; GPVI ELISA Kit; GPVI_HUMAN ELISA Kit; MGC138168 ELISA Kit; Platelet collagen receptor ELISA Kit; Platelet glycoprotein VI ELISA Kit; Platelet glycoprotein VI precursor ELISA Kit
缩写:GP6
Uniprot No.:Q9HCN6
种属:Homo sapiens (Human)
样本类型:serum, plasma, tissue homogenates
检测范围:46.88 pg/mL-3000 pg/mL
灵敏度:11.72 pg/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Blood Coagulation
测定原理:quantitative
测定方法:Sandwich
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
Inter-assay Precision (Precision between assays): CV%<10% | |||||||
Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human GP6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
Sample | Serum(n=4) | ||||||
1:1 | Average % | 97 | |||||
Range % | 88-101 | ||||||
1:2 | Average % | 101 | |||||
Range % | 97-105 | ||||||
1:4 | Average % | 96 | |||||
Range % | 87-100 | ||||||
1:8 | Average % | 104 | |||||
Range % | 97-108 |
回收率:
The recovery of human GP6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
Sample Type | Average % Recovery | Range | |||||
Serum (n=5) | 99 | 93-104 | |||||
EDTA plasma (n=4) | 97 | 90-101 | |||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/mlOD1OD2AverageCorrected30002.6682.5452.6072.46915001.9471.9151.9311.7937501.1131.1741.1441.0063750.6950.6880.6920.554187.50.3920.3850.3890.25193.750.3150.3070.3110.17346.880.2150.2220.2190.08100.1400.1360.138 |
货期:3-5 working days
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