首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠磷脂酰肌醇蛋白聚糖3(GPC-3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的GPC3含量。GPC3是一种糖基磷脂酰肌醇锚定蛋白，在正常肝脏组织中几乎不表达，在肝癌等多种肿瘤中高表达。研究机制上，其可促进肿瘤细胞增殖、迁移等。因其在肿瘤组织中的特异性，成为极具潜力的肿瘤治疗靶点，为肝癌等治疗提供了新思路。试剂盒检测范围为3.12 ng/mL-200 ng/mL，适用于小鼠模型中的GPC-3表达水平监测、肿瘤相关信号通路研究以及体外药物干预效果评估等科研场景，为肝脏疾病机制研究及生物标志物筛选提供高效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Gpc3 ELISA Kit; Glypican-3 [Cleaved into: Glypican-3 alpha subunit; Glypican-3 beta subunit] ELISA Kit
缩写：GPC3
Uniprot No.：Q8CFZ4
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse GPC-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 92 | |||||
| Range % | 84-95 | ||||||
| 1:2 | Average % | 94 | |||||
| Range % | 84-98 | ||||||
| 1:4 | Average % | 94 | |||||
| Range % | 85-97 | ||||||
| 1:8 | Average % | 90 | |||||
| Range % | 84-94 | ||||||
回收率：
| The recovery of mouse GPC-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 99 | 91-103 | |||||
| EDTA plasma (n=4) | 95 | 90-99 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected2002.1732.2022.1882.0501001.6481.6831.6661.528500.9360.9110.9240.786250.5490.5400.5450.40712.50.3010.3230.3120.1746.250.2450.2510.2480.1103.120.1780.1810.1800.04200.1370.1390.138 | |||||||
货期：3-5 working days
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