首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人磷脂酰肌醇蛋白聚糖6(GPC6)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的GPC6含量。GPC6 是一个靶点。它在人体生理和病理过程中有重要作用。研究发现其参与细胞信号传导等机制。围绕它探究疾病发生发展机制、寻找有效治疗靶点是当前研究方向，有望为相关疾病如特定癌症等的诊治带来新突破。试剂盒检测范围为0.312 ng/mL-20 ng/mL，适用于肿瘤生物学研究、细胞信号转导机制探索、骨骼发育相关实验等科研领域本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：A Kit; Glypican 6 [Precursor] ELISA Kit; Glypican proteoglycan 6 ELISA Kit; Gpc6 ELISA Kit; GPC6_HUMAN ELISA Kit; MGC126288 ELISA Kit; OMIMD1 ELISA Kit; PRO705 ELISA Kit; Secreted glypican 6 ELISA Kit; Secreted glypican-6 ELISA Kit; UNQ369 ELISA Kit
缩写：GPC6
Uniprot No.：Q9Y625
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GPC6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 87 | |||||
| Range % | 82-91 | ||||||
| 1:2 | Average % | 94 | |||||
| Range % | 89-98 | ||||||
| 1:4 | Average % | 107 | |||||
| Range % | 103-110 | ||||||
| 1:8 | Average % | 110 | |||||
| Range % | 104-114 | ||||||
回收率：
| The recovery of human GPC6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 91-99 | |||||
| EDTA plasma (n=4) | 103 | 98-108 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected202.4432.4032.4232.271101.6921.7021.6971.54551.2181.2681.2431.0912.50.8540.8650.8600.7081.250.6020.6120.6070.4550.6250.4130.4030.4080.2560.3120.2740.2680.2710.11900.1530.1500.152 | |||||||
货期：3-5 working days
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