首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠丙氨酸氨基转移酶(ALT)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的GPT含量。试剂盒检测范围为3.12 mIU/mL-200 mIU/mL，该产品适用于实验室中肝毒性评估、药物代谢研究、疾病动物模型建立等场景，例如可通过检测不同处理条件下肝组织匀浆或血清中的ALT水平变化，评估化学物质、药物或病理状态对肝脏功能的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Gpt ELISA Kit; Aat1 ELISA Kit; Gpt1 ELISA Kit; Alanine aminotransferase 1 ELISA Kit; ALT1 ELISA Kit; EC 2.6.1.2 ELISA Kit; Glutamate pyruvate transaminase 1 ELISA Kit; GPT 1 ELISA Kit; Glutamic--alanine transaminase 1 ELISA Kit; Glutamic--pyruvic transaminase 1 ELISA Kit
缩写：GPT
Uniprot No.：P25409
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：3.12 mIU/mL-200 mIU/mL
灵敏度：0.78 mIU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat ALT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 92-110 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of rat ALT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 96 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| mIU/mlOD1OD2AverageCorrected2002.8142.9112.8632.7781002.4662.3552.4112.326501.6591.5641.6121.527250.8530.8120.8330.74812.50.4690.4420.4560.3716.250.2430.2260.2350.1503.120.1490.1410.1450.06000.0880.0820.085 | ||||||
货期：3-5 working days
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