首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人颗粒体蛋白 (GRN)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的GRN含量。GRN即颗粒溶素基因，其表达的颗粒溶素是一种具有抗菌、抗病毒及抗肿瘤活性的蛋白。研究机制上，它可通过形成孔道破坏病原体膜结构，还能诱导肿瘤细胞凋亡、调节免疫反应等，在感染性疾病和癌症治疗研究中颇具潜力。试剂盒检测范围为7.8 pg/mL-500 pg/mL，适用于科研场景中GRN表达水平的动态监测，例如探究神经退行性疾病模型、炎症相关细胞实验或药物干预研究中GRN的调控机制，为疾病分子机制探索及生物标志物分析提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Acrogranin ELISA Kit; CLN11 ELISA Kit; GEP ELISA Kit; GP88 ELISA Kit; Granulin A ELISA Kit; Granulin B ELISA Kit; Granulin C ELISA Kit; Granulin D ELISA Kit; Granulin E ELISA Kit; Granulin epithelin ELISA Kit; Granulin F ELISA Kit; Granulin G ELISA Kit; Granulin-7 ELISA Kit; Granulins ELISA Kit; GRN ELISA Kit; GRN_HUMAN ELISA Kit; PC cell derived growth factor ELISA Kit; PCDGF ELISA Kit; PEPI ELISA Kit; PGRN ELISA Kit; Proepithelin ELISA Kit; Progranulin ELISA Kit
缩写：GRN
Uniprot No.：P28799
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GRN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1000 | Average % | 84 | |||||
| Range % | 81-86 | ||||||
| 1:2000 | Average % | 85 | |||||
| Range % | 82-89 | ||||||
| 1:4000 | Average % | 104 | |||||
| Range % | 101-107 | ||||||
| 1:8000 | Average % | 89 | |||||
| Range % | 85-93 | ||||||
回收率：
| The recovery of human GRN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 95 | 92-98 | |||||
| EDTA plasma (n=4) | 105 | 101-109 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
 pg/mlOD1OD2AverageCorrected5001.9432.1052.0241.9422501.5661.5301.5481.4661251.0191.1111.0650.98362.50.6910.7050.6980.61631.20.4100.4310.4210.33915.60.2560.2410.2490.1677.80.1780.1680.1730.09100.0810.0830.082 | |||||||
货期：3-5 working days
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